SARS-CoV-2 Vaccine-Induced T-Cell Response after Three Doses in People Living with HIV on Antiretroviral Therapy Compared to Seronegative Controls (CTN 328 COVAXHIV Study).
SARS-CoV-2 Vaccine-Induced T-Cell Response after Three Doses in People Living with HIV on Antiretroviral Therapy Compared to Seronegative Controls (CTN 328 COVAXHIV Study).
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DOI：
10.3390/v15020575
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发表时间：
2023-02-19
期刊：
影响因子：
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通讯作者：
Jenabian MA
中科院分区：
文献类型：
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作者：
Alexandrova Y;Yero A;Mboumba Bouassa RS;Comeau E;Samarani S;Brumme ZL;Hull M;Crawley AM;Langlois MA;Angel JB;Cooper CL;Needham J;Lee T;Singer J;Anis AH;Costiniuk CT;Jenabian MA
艾滋病毒感染者（PLWH）可能对某些疫苗的免疫原性较差，包括产生免疫记忆的能力。在此，我们评估了PLWH与未感染对照组中三剂SARS-CoV-2疫苗后的T细胞免疫原性。从38名接受抗逆转录病毒治疗的PLWH和24名年龄匹配的HIV阴性对照中采集血液，分别在接种前和第1/2/3剂SARS-CoV-2疫苗后，既往无SARS-CoV-2感染。流式细胞术用于评估SARS-CoV-2-Spike-peptide刺激后的离体T细胞免疫表型和细胞内肿瘤坏死因子（TNF）-α/干扰素（IFN）-γ/白细胞介素（IL）-2。配对变量采用Wilcoxon符号秩检验，未配对变量采用Mann-Whitney检验。在PLWH中，刺突特异性CD 4 T细胞频率在第2次给药后趋于稳定，第3次给药后PLWH和未感染对照之间多功能SARS-CoV-2特异性T细胞比例无显著差异。第3次给药后，PLWH的TNFα+ CD 4 T细胞频率高于血清阴性受试者，IFNγ+ CD 8 T细胞频率低于血清阴性受试者。无论HIV状态如何，在第3次给药后观察到幼稚、调节和PD 1 + T细胞频率增加。总之，两剂SARS-CoV-2疫苗在PLWH中诱导了强有力的T细胞免疫应答，在第3剂后维持，在第3剂后PLWH和未感染对照之间的多功能SARS-CoV-2特异性T细胞比例没有显著差异。
People living with HIV (PLWH) may be at risk for poor immunogenicity to certain vaccines, including the ability to develop immunological memory. Here, we assessed T-cell immunogenicity following three SARS-CoV-2 vaccine doses in PLWH versus uninfected controls. Blood was collected from 38 PLWH on antiretroviral therapy and 24 age-matched HIV-negative controls, pre-vaccination and after 1st/2nd/3rd dose of SARS-CoV-2 vaccines, without prior SARS-CoV-2 infection. Flow cytometry was used to assess ex vivo T-cell immunophenotypes and intracellular Tumor necrosis factor (TNF)-α/interferon(IFN)-γ/interleukin(IL)-2 following SARS-CoV-2-Spike-peptide stimulation. Comparisons were made using Wilcoxon signed-rank test for paired variables and Mann–Whitney for unpaired. In PLWH, Spike-specific CD4 T-cell frequencies plateaued post-2nd dose, with no significant differences in polyfunctional SARS-CoV-2-specific T-cell proportions between PLWH and uninfected controls post-3rd dose. PLWH had higher frequencies of TNFα+CD4 T-cells and lower frequencies of IFNγ+CD8 T-cells than seronegative participants post-3rd dose. Regardless of HIV status, an increase in naive, regulatory, and PD1+ T-cell frequencies was observed post-3rd dose. In summary, two doses of SARS-CoV-2 vaccine induced a robust T-cell immune response in PLWH, which was maintained after the 3rd dose, with no significant differences in polyfunctional SARS-CoV-2-specific T-cell proportions between PLWH and uninfected controls post-3rd dose.
影响因子：
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作者：
Bayry, Jagadeesh
通讯作者：
Bayry, Jagadeesh