TaMDAR6 acts as a negative regulator of plant cell death and participates indirectly in stomatal regulation during the wheat stripe rust-fungus interaction
TaMDAR6 acts as a negative regulator of plant cell death and participates indirectly in stomatal regulation during the wheat stripe rust-fungus interaction
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TaMDAR6 作为植物细胞死亡的负调节因子，并在小麦条锈病-真菌相互作用过程中间接参与气孔调节
DOI：
10.1111/ppl.12355
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发表时间：
2016-01-01
影响因子：
6.4
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
--
作者：
Abou-Attia, Mohamed Awaad;Wang, Xiaojie;Kang, Zhensheng
我们从小麦中发现了一个新的单脱氢抗坏血酸还原酶基因，命名为TaMDAR6，该基因受小麦-条锈菌的差异影响。小麦(PST)互作。TaMDAR6是Bax基因和PST引发的植物细胞死亡(PCD)的负调控因子。在亲和的小麦-PST互作过程中，TaMDAR6的转录水平显著上调，这表明TaMDAR6可能与植物的感病有关。此外，在感染PST的TaMDAR6击倒植株中，显著诱导了H_2O_2的产生和PCD，并显著减少了初始病原菌的发展。因此，TaMDAR6的抑制增强了小麦对PST的抗性。此外，TaMDAR6在不亲和互作过程中的抑制会导致气孔形态的改变，从而导致气孔识别不良，从而降低侵染效率。在不亲和互作过程中，TaMDAR6击倒植株中形成气孔下小泡的侵染部位的百分比下降，这可能是由于ROS积累的增加，这可能激活了其他对气孔下小泡形成有负面影响的抗性机制。因此，TaMDAR6可以被认为是PCD和小麦对PST的防御的负调控因子。
We identified a new monodehydroascorbate reductase (MDAR) gene from wheat, designated TaMDAR6, which is differentially affected by wheat-Puccinia striiformis f. sp. tritici (Pst) interactions. TaMDAR6 is a negative regulator of plant cell death (PCD) triggered by the Bax gene and Pst. Transcript levels of TaMDAR6 are significantly upregulated during a compatible wheat-Pst interaction, indicating that TaMDAR6 may contribute to plant susceptibility. In addition, H2O2 production and PCD are significantly induced and initial pathogen development is significantly reduced in the TaMDAR6 knocked-down plants upon Pst infection. Thus, the suppression of TaMDAR6 enhances wheat resistance to Pst. Besides, the suppression of TaMDAR6 during an incompatible interaction induces a change in the morphology of stomata, which leads to poor stoma recognition and as a consequence to reduced infection efficiency. The percentage of infection sites that develop substomatal vesicles decreases in the TaMDAR6 knocked-down plants during the incompatible interaction presumably due to the increase in ROS accumulation, which is likely to activate other resistance mechanisms that have a negative effect on substomatal vesicle formation. TaMDAR6 can therefore be considered a negative regulator of PCD and of wheat defense to Pst.