Mechanical strain tightly controls fibroblast growth factor-2 release from cultured human vascular smooth muscle cells
Mechanical strain tightly controls fibroblast growth factor-2 release from cultured human vascular smooth muscle cells
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DOI：
10.1161/01.res.80.1.28
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发表时间：
1997-01-01
影响因子：
20.1
通讯作者：
Lee, RT
中科院分区：
文献类型：
--
作者：
Cheng, GC;Briggs, WH;Lee, RT
尽管成纤维细胞生长因子-2参与了血管损伤的反应，但细胞变形在释放成纤维细胞生长因子-2中的作用尚不完全清楚。为了验证机械应变严格控制成纤维细胞生长因子-2释放的假设，一种新的装置被用来对人血管平滑肌细胞施加均匀和均匀的双轴应变。随着培养循环次数的增加(14%，1 Hz)，1、9、90个循环的细胞分别释放了0.55+/-0.06%、2.9+/-0.3%和5.5+/-1.3%的成纤维细胞生长因子-2(对照组为0.00+/-0.40%，P
Although fibroblast growth factor-2 (FGF-2) participates in the response to vascular injury, the role of cellular deformation in FGF-2 release is incompletely understood. To test the hypothesis that mechanical strain tightly controls FGF-2 release, a novel device was used to impose homogeneous and uniform biaxial strain to human vascular smooth muscle cells. Release of FGF-2 increased with the number of cycles of strain (14%, 1 Hz); 1; 9, and 90 cycles of strain, respectively, released 0.55+/-0.06%, 2.9+/-0.3%, and 5.5+/-1.3% of the total cellular FGF-2 (versus 0.00+/-0.40% for control, P