Naphthoquinone enhances antigen-related airway inflammation in mice
Naphthoquinone enhances antigen-related airway inflammation in mice
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DOI：
10.1183/09031936.00033106
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发表时间：
2007-02-01
影响因子：
24.3
通讯作者：
Kumagai, Y.
中科院分区：
文献类型：
--
作者：
Inoue, K.;Takano, H.;Kumagai, Y.
目前的作者先前已经证明，柴油机排气颗粒（DEP）增强小鼠抗原相关的气道炎症。此外，最近的一项研究表明，DEP中的有机化学物质，而不是它们的碳质核，是加重气道炎症的重要因素。然而，DEP中负责增强模型效果的成分仍有待鉴定，目前作者研究了DEP的可提取化合物之一萘醌（NO）对小鼠抗原相关气道炎症、细胞因子蛋白的局部表达和抗原特异性免疫球蛋白（1g）产生的影响。肺暴露于NO剂量依赖性地加重抗原相关的气道炎症，其特征在于气道周围的嗜酸性粒细胞和淋巴细胞的浸润和支气管上皮中杯状细胞的增加。与单独暴露于抗原或NO相比，联合暴露于NO和抗原增强了局部白细胞介素（IL）-4，IL-5，嗜酸性粒细胞趋化因子，巨噬细胞趋化蛋白-1和角质形成细胞趋化因子的表达。NO对抗原特异性IgG（1）和IgG（2a）的产生也有一定的辅助作用，这一结果首次证明了萘醌在体内可增强抗原相关的气道炎症，并在一定程度上参与了柴油机尾气颗粒物毒性的发病过程。
The current authors have previously demonstrated that diesel exhaust particles (DEP) enhance antigen-related airway inflammation in mice. Furthermore, a recent study has shown that organic chemicals in DEP, rather than their carbonaceous nuclei, are important contributors to the aggravating effects of airway inflammation. However, the components in DEP responsible for the enhancing effects on the model remain to be identified.The current authors investigated the effects of naphthoquinone (NO), one of the extractable chemical compounds of DEP, on antigen-related airway inflammation, local expression of cytokine proteins, and antigen-specific immunoglobulin (1g) production in mice. Pulmonary exposure to NO dose-dependently aggravated antigen-related airway inflammation, as characterised by infiltration of eosinophils and lymphocytes around the airways and an increase in goblet cells in the bronchial epithelium. Combined exposure to NO and antigen enhanced the local expression of interleukin (IL)-4, IL-5, eotaxin, macrophage chemoattractant protein-1 and keratinocyte chernoattractant, compared with exposure to antigen or NO alone. Also, NO exhibited adjuvant activity for the antigen-specific production of IgG(1) and IgG(2a).These results provide the first experimental evidence that naphthoquinone can enhance antigen-related airway inflammation in vivo, and that naphthoquinone can, to some extent, partly play a role in the pathogenesis of diesel exhaust particle toxicity on the condition.