Molecular characterization of axonemal proteins and signaling molecules responsible for chemoattractant-induced sperm activation in Ciona intestinalis
Molecular characterization of axonemal proteins and signaling molecules responsible for chemoattractant-induced sperm activation in Ciona intestinalis
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DOI：
10.1002/cm.20258
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发表时间：
2008-03-01
影响因子：
--
通讯作者：
Inaba, Kazuo
中科院分区：
文献类型：
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作者：
Hozumi, Akiko;Padma, Potturi;Inaba, Kazuo
精子在受精过程中会发生剧烈的生理变化。在海鞘中，一种名为SAAF的卵源物质诱导精子激活和对卵的趋化性。为了阐明这些现象背后的分子机制，整个精子蛋白的SAAF处理前后进行了分析，通过二维凝胶电泳。通过比较激活前后的斑点图案，我们发现了12个改变等电点的蛋白质。7个蛋白质被证明是轴丝蛋白和其他被认为是非轴丝组分。通过质谱分析发现，在精子激活过程中，轴丝的几个亚结构发生了等电点的变化，其中包括外臂和内臂动力蛋白的WD重复中间链和辐射辐蛋白LRR 37，以及具有Armadillo重复序列或SMC结构域的新的轴丝蛋白。细胞信号分子如14-3-3蛋白、Skp 1和VCP/p97在精子激活时也显示出等电变化。这些结果显示了受精时精子激活的信号机制的综合特征，也为纤毛和鞭毛运动的调节提供了新的线索。
Spermatozoa undergo dramatic physiological changes at fertilization. In the ascidian Ciona intestinalis, an egg-derived substance named SAAF induces both sperm activation and chemotaxis to the egg. To elucidate the molecular mechanism underlying these phenomena, whole sperm proteins before and after SAAF-treatment were analyzed by two-dimensional gel electrophoresis. By comparison of spot patterns before and after activation, we found twelve proteins that changed the isoelectric points. Seven proteins were shown to be axonemal proteins and others were suggested to be non-axonemal components. Analysis of these proteins by MS-based proteomic system revealed that components of several substructures of the axonemes underwent the changes in isoelectric point at sperm activation, including WD-repeat intermediate chains of outer and inner arm dyneins and a radial spoke protein LRR37, as well as novel axonemal proteins with armadillo repeats or SMC domain. Molecules for cell signaling such as 14-3-3 proteins, Skp1 and VCP/p97 also showed isoelectric changes at sperm activation. These results show a comprehensive feature for signaling mechanism of the activation of spermatozoa at fertilization and also shed new lights on the regulation of ciliary and flagellar movements.