Identification and characterization of a juvenile hormone response element and its binding proteins
Identification and characterization of a juvenile hormone response element and its binding proteins
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DOI：
10.1074/jbc.m704595200
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发表时间：
2007-12-28
影响因子：
4.8
通讯作者：
Palli, Subba R.
中科院分区：
文献类型：
--
作者：
Li, Yiping;Zhang, Zhaolin;Palli, Subba R.
保幼激素（JH）调节昆虫的多种发育和生理过程。比较JH诱导的基因在果蝇，果蝇，L57细胞和蜜蜂，意大利蜜蜂的微阵列数据，确定了16个基因，在这两个物种中诱导。对这16个D.黑腹果蝇基因DmJHRE 1（D.黑腹JH反应元件1）。在L57细胞中，由DmJHRE 1调控的报告基因被JH III诱导。鉴定了与DmJHRE 1结合的两种蛋白质（FKBP 39和Chd 64）。FKBP39和Chd64双链RNA抑制JH III诱导DmJHRE 1调控的报告基因。FKBP39和Chd64蛋白在酵母中表达结合DmJHRE 1。双杂交和pulldown分析表明，这两个蛋白相互作用，以及与蜕皮激素受体，ultraspicacle，和甲氧普烯耐受蛋白。FKBP 39和Chd 64蛋白的发育表达谱和JH对mRNA的诱导以及它们与已知参与JH（甲氧普仑耐受蛋白）和蜕皮甾类作用（蜕皮激素受体和超气门）的蛋白的相互作用表明，这些蛋白可能在JH和蜕皮甾类之间的串扰中起重要作用。
Juvenile hormones (JH) regulate a wide variety of developmental and physiological processes in insects. Comparison of microarray data on JH-induced genes in the fruit fly, Drosophila melanogaster, L57 cells and in the honey bee, Apis mellifera, identified 16 genes that are induced in both species. Analysis of promoter regions of these 16 D. melanogaster genes identified DmJHRE1 (D. melanogaster JH response element 1). In L57 cells, the reporter gene regulated by DmJHRE1 was induced by JH III. Two proteins (FKBP39 and Chd64) that bind to DmJHRE1 were identified. FKBP39 and Chd64 double-stranded RNA inhibited JH III induction of a reporter gene regulated by DmJHRE1. FKBP39 and Chd64 proteins expressed in yeast bound to DmJHRE1. Two-hybrid and pulldown assays showed that these two proteins interact with each other as well as with ecdysone receptor, ultraspiracle, and methoprene- tolerant protein. Developmental expression profiles and JH induction of mRNA for FKBP39 and Chd64 proteins and their interaction with proteins known to be involved in both JH (methoprene-tolerant protein) and ecdysteroid action (ecdysone receptor and ultraspiracle) suggest that these proteins probably play important roles in cross- talk between JH and ecdysteroids.