TGF-β1 favors the development of fast type identity during soleus muscle regeneration
TGF-β1 favors the development of fast type identity during soleus muscle regeneration
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DOI：
10.1007/s10974-005-9014-9
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发表时间：
2006-02-01
影响因子：
2.7
通讯作者：
Ferry, Arnaud
中科院分区：
文献类型：
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作者：
Noirez, Philippe;Torres, Sandra;Ferry, Arnaud
已知转化生长因子-β 1（TGF-β 1）在个体发育期间在发育快肌纤维的环境中表达。在本研究中，我们研究了施用TGF-β 1或中和TGF-β 1抗体对大鼠再生骨骼肌中快型表型诱导的影响。快和慢肌球蛋白重链（MHC）亚型的表达进行了研究，在3周和6周后肌毒治疗使用蛋白电泳。还原位测量了肌肉收缩特性。结果表明，在再生的慢比目鱼肌中单独注射TGF-β_1可增加快MHC-2x/d和MHC-2a的表达，降低慢MHC-1的表达（P < 0.05）。收缩时强直融合程度明显降低（P < 0.05）。相反，注射TGF-β 1中和抗体后，再生快肌中MHC-2a和MHC-1的表达增加（P < 0.05）。总之，当缓慢的肌肉在TGF-β 1水平增加的情况下再生时，它诱导了向较慢的MHC表型和收缩特征的转变。相反，在再生快肌中TGF-β 1的中和诱导了向不太快的MHC表达的转变。总之，这些结果表明，TGF-β 1影响骨骼肌再生过程中快速肌肉型模式的某些方面。
Transforming growth factor-beta 1 (TGF-beta 1) is known to be expressed in the environment of developing fast muscle fibres during ontogenesis. In the present study, we have examined effects of administration of either TGF-beta 1 or neutralizing TGF-beta 1 antibody on the induction of fast type phenotype in regenerating skeletal muscles in rats. Expressions of fast and slow myosin heavy chain (MHC) isoforms were studied using protein electrophoresis, at 3 and 6 weeks after myotoxic treatment. Muscle contractile properties were also measured in situ. The results have shown that a single injection of TGF-beta 1 into the regenerating slow soleus muscle increased the expression of fast MHC-2x/d and MHC-2a and decreases that of slow MHC-1 (P < 0.05). Moreover, it reduced the degree of tetanic fusion during contraction (P < 0.05). Conversely, injection of neutralizing antibody against TGF-beta 1 into the regenerating fast EDL muscle increased the expression of MHC-2a and MHC-1 (P < 0.05). In conclusion, when the slow muscle was regenerating in the presence of an increased level of TGF-beta 1, it induced a shift to a less slow MHC phenotype and contractile characteristics. Conversely, neutralization of TGF-beta 1 in the regenerating fast muscle induced a shift to a less fast MHC expression. Together these results suggest that TGF-beta 1 influences some aspects of fast muscle-type patterning during skeletal muscle regeneration.