Synchronized mesenchymal cell polarization and differentiation shape the formation of the murine trachea and esophagus.
Synchronized mesenchymal cell polarization and differentiation shape the formation of the murine trachea and esophagus.
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DOI：
10.1038/s41467-018-05189-2
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发表时间：
2018-07-19
影响因子：
16.6
通讯作者：
Morimoto M
中科院分区：
文献类型：
--
作者：
Kishimoto K;Tamura M;Nishita M;Minami Y;Yamaoka A;Abe T;Shigeta M;Morimoto M
管的形态发生是内部器官发育的必要条件，但调节管形状的机制尚不清楚。在这里，我们展示了不同的机制调节小鼠气管的长度和直径。首先，我们发现气管的发育是通过连续的伸长和扩张过程进行的。这始于平滑肌（SM）祖细胞的同步径向极化，向内高尔基体位移调节管伸长，由间质Wnt5a-Ror2信号控制。这种径向极化引导SM祖细胞向上皮迁移，由此产生的上皮下形态发生支持管向前后轴延伸。这种径向极化也调节食管伸长。随后，软骨的发育有助于扩大管直径，从而推动上皮细胞的重塑，以确定有效呼吸的最佳管腔形状。这些发现提示了一种策略，即直接器官的小管形成是由上皮下细胞极化和环状软骨发育驱动的。气管的发育是由于管的形态发生引起的，但这是如何调节的尚不清楚。在这里，作者发现平滑肌祖细胞的极化控制小鼠气管发育，激活非规范Wnt信号，随后是上皮下形态发生和环状软骨发育。
Tube morphogenesis is essential for internal-organ development, yet the mechanisms regulating tube shape remain unknown. Here, we show that different mechanisms regulate the length and diameter of the murine trachea. First, we found that trachea development progresses via sequential elongation and expansion processes. This starts with a synchronized radial polarization of smooth muscle (SM) progenitor cells with inward Golgi-apparatus displacement regulates tube elongation, controlled by mesenchymal Wnt5a-Ror2 signaling. This radial polarization directs SM progenitor cell migration toward the epithelium, and the resulting subepithelial morphogenesis supports tube elongation to the anteroposterior axis. This radial polarization also regulates esophageal elongation. Subsequently, cartilage development helps expand the tube diameter, which drives epithelial-cell reshaping to determine the optimal lumen shape for efficient respiration. These findings suggest a strategy in which straight-organ tubulogenesis is driven by subepithelial cell polarization and ring cartilage development. Tracheal development arises due to tube morphogenesis but how this is regulated is unclear. Here, the authors identify polarization of smooth muscle progenitors as controlling murine tracheal development, activating noncanonical Wnt signaling followed by subepithelial morphogenesis and ring cartilage development.
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影响因子：
11.8
作者：
Kim HY;Pang MF;Varner VD;Kojima L;Miller E;Radisky DC;Nelson CM
通讯作者：
Nelson CM
DOI：
10.1073/pnas.0902274106
发表时间：
2009-09-22
影响因子：
11.1
作者：
Harris-Johnson, Kelley S.;Domyan, Eric T.;Sun, Xin
通讯作者：
Sun, Xin
DOI：
10.1038/nrm3176
发表时间：
2011-08-23
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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影响因子：
2.7
作者：
Li, CG;Xiao, J;Minoo, P
通讯作者：
Minoo, P
影响因子：
2.7
作者：
Cervantes S;Yamaguchi TP;Hebrok M
通讯作者：
Hebrok M