Rapid Two-Millisecond Interrogation of Electrochemical, Aptamer-Based Sensor Response Using Intermittent Pulse Amperometry
Rapid Two-Millisecond Interrogation of Electrochemical, Aptamer-Based Sensor Response Using Intermittent Pulse Amperometry
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DOI：
10.1021/acssensors.8b00278
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发表时间：
2018-06-01
期刊：
影响因子：
8.9
通讯作者：
White, Ryan J.
中科院分区：
文献类型：
--
作者：
Santos-Cancel, Mirelis;Lazenby, Robert A.;White, Ryan J.
在本文中，我们采用间歇脉冲安培法（IPA）技术来询问电化学，基于适体（E-AB）传感器表面的平衡和动力学目标结合，实现了快至2毫秒的时间分辨率。E-AB传感器包括用柔性核酸适体修饰的电极表面，该核酸适体连接在具有氧化还原活性分子的3'端。靶标的引入改变了核酸的构象和柔韧性，从而改变了附加的氧化还原分子的电荷转移速率。通常，这类传感器内电荷转移速率的变化是通过伏安法监测的。在这里，我们证明了IPA的使用可以检测到电荷转移率（即电流）的变化
In this manuscript, we employ the technique intermittent pulse amperometry (IPA) to interrogate equilibrium and kinetic target binding to the surface of electrochemical, aptamer-based (E-AB) sensors, achieving as fast as 2 ms time resolution. E-AB sensors comprise an electrode surface modified with a flexible nucleic acid aptamer tethered at the 3'-terminus with a redox-active molecule. The introduction of a target changes the conformation and flexibility of the nucleic acid, which alters the charge transfer rate of the appended redox molecule. Typically, changes in charge transfer rate within this class of sensor are monitored via voltammetric methods. Here, we demonstrate that the use of IPA enables the detection of changes in charge transfer rates (i.e., current) at times