Hippocampal CA1 pyramidal neurons exhibit type 1 phase-response curves and type 1 excitability.
Hippocampal CA1 pyramidal neurons exhibit type 1 phase-response curves and type 1 excitability.
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DOI：
10.1152/jn.00721.2012
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发表时间：
2013-06
影响因子：
2.5
通讯作者：
Shuoguo Wang;Maximilian M. Musharoff;C. Canavier;S. Gasparini
中科院分区：
文献类型：
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作者：
Shuoguo Wang;Maximilian M. Musharoff;C. Canavier;S. Gasparini
神经元的相位重置特性决定了它们作为积分器（类型1）和谐振器（类型2）的功能，以及它们的同步倾向。我们介绍了一种新的偏差校正方法，通过两种独立的方法来估计无穷小相位重置曲线（iPRC）并确认海马体锥体CA1神经元的1型兴奋性。首先，使用去极化脉冲诱发的prc仅包括进展，与类型1一致。其次，频率/电流（f/I）图没有显示最小频率，再次与类型1一致。从f/I数据得出的峰间直方图中没有共振峰，也证实了1型兴奋性。PRC偏差校正假设噪声相位重置的分布被截断，因为输入不能将尖峰推进到输入之前的某个时间点（因果限制），并且通过计算相位重置作为未截断分布的平均值，成功地消除了响应于零幅度输入的零PRC延迟的统计偏差。去极化的PRC在后期达到峰值，并在周期结束时降至零，而响应超极化的延迟则单调增加。偏置校正不影响这种形状上的差异，这是由于因果限制掩盖了去极化的iPRC，而不是超极化。我们的研究结果表明，弱周期超极化驱动理论上可以在任何阶段夹带CA1锥体神经元，但强激励会优先锁相，并具有零滞后。
Phase-resetting properties of neurons determine their functionality as integrators (type 1) vs. resonators (type 2), as well as their synchronization tendencies. We introduce a novel, bias-correction method to estimate the infinitesimal phase-resetting curve (iPRC) and confirm type 1 excitability in hippocampal pyramidal CA1 neurons in vitro by two independent methods. First, PRCs evoked using depolarizing pulses consisted only of advances, consistent with type 1. Second, the frequency/current (f/I) plots showed no minimum frequency, again consistent with type 1. Type 1 excitability was also confirmed by the absence of a resonant peak in the interspike interval histograms derived from the f/I data. The PRC bias correction assumed that the distribution of noisy phase resetting is truncated, because an input cannot advance a spike to a point in time before the input (the causal limit) and successfully removed the statistical bias for delays in the null PRC in response to zero-magnitude input by computing the phase resetting as the mean of the untruncated distribution. The PRC for depolarization peaked at late phases and decreased to zero by the end of the cycle, whereas delays observed in response to hyperpolarization increased monotonically. The bias correction did not affect this difference in shape, which was due instead to the causal limit obscuring the iPRC for depolarization but not hyperpolarization. Our results suggest that weak periodic hyperpolarizing drive can theoretically entrain CA1 pyramidal neurons at any phase but that strong excitation will preferentially phase-lock them with zero time lag.