Tumour-necrosis factor from the rabbit. II. Production by monocytes.
Tumour-necrosis factor from the rabbit. II. Production by monocytes.
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DOI：
10.1038/bjc.1978.203
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发表时间：
1978-08
影响因子：
8.8
通讯作者：
Matthews, N
中科院分区：
文献类型：
--
作者：
Matthews, N
正常兔血单核细胞在体外对多种细胞系具有细胞毒性。细胞毒性细胞包含在粘附于塑料的富含单核细胞的部分中。单核细胞富集级分的上清液具有与亲本细胞相同的细胞毒性特异性。细胞毒性因子在50%饱和硫酸铵溶液中沉淀，在凝胶过滤上是不均匀的，摩尔浓度为重量范围30- 50，000 u.根据特异性和分子特征，这种细胞毒性因子与兔肿瘤坏死因子（TNF）非常相似，表明TNF或一种密切相关的因子是单核吞噬细胞的正常产物。
Mononuclear cells from normal rabbit blood were cytotoxic to a number of cell lines in vitro. The cytotoxic cells were contained in the monocyte-enriched fraction adherent to plastic. Supernatants of the monocyte-enriched fraction had the same cytotoxic specificity as the parent cells. The cytotoxic factor precipitated in 50% saturated ammonium sulphate solution and on gel filtration was heterogeneous with a mol.-wt. range of 30--50,000 u. On the basis of specificity and molecular characteristics, this cytotoxic factor closely resembles rabbit tumour-necrosis factor (TNF) suggesting that TNF or a closely related factor is a normal product of mononuclear phagocytes.