Comparative analysis of the inverted repeat of a chalcone synthase pseudogene between yellow soybean and seed coat pigmented mutants
Comparative analysis of the inverted repeat of a chalcone synthase pseudogene between yellow soybean and seed coat pigmented mutants
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DOI：
10.1270/jsbbs.63.384
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发表时间：
2013-12-01
期刊：
影响因子：
2.4
通讯作者：
Kuroda, Tomohisa
中科院分区：
文献类型：
--
作者：
Senda, Mineo;Nishimura, Satsuki;Kuroda, Tomohisa
在大豆中，I基因抑制整个种皮的色素沉着，导致黄色种子。据认为，这种抑制种皮色素沉着是由于天然存在的RNA沉默的查耳酮合酶基因（CHS沉默）。在收获的黄色种子中，完全着色的种子的比例很低。这些种皮色素突变体是由黄大豆的I基因自发隐性突变产生的。I基因的候选者GmIRCHS含有CHS假基因（pseudoCHS3）的完美反向重复（IR），并且GmIRCHS的转录物形成可能触发CHS沉默的双链CHS RNA。CHS基因ICHS 1位于GmIRCHS下游680 bp处。这里，GmIRCHS ICHS 1簇在不同来源的scp突变体中进行了比较。在这些突变体中，簇中的序列趋异导致至少在pseudoCHS3区域中GmIRCHS的完全或部分丧失。该结果与pseudoCHS3的IR足以诱导CHS沉默的观点一致，并且进一步支持GmIRCHS是I基因。
In soybean, the I gene inhibits pigmentation over the entire seed coat, resulting in yellow seeds. It is thought that this suppression of seed coat pigmentation is due to naturally occurring RNA silencing of chalcone synthase genes (CHS silencing). Fully pigmented seeds can be found among harvested yellow seeds at a very low percentage. These seed coat pigmented (scp) mutants are generated from yellow soybeans by spontaneous recessive mutation of the I gene. A candidate for the I gene, GmIRCHS, contains a perfect inverted repeat (IR) of a CHS pseudogene (pseudoCHS3) and transcripts of GmIRCHS form a double-stranded CHS RNA that potentially triggers CHS silencing. One CHS gene, ICHS1, is located 680 bp downstream of GmIRCHS. Here, the GmIRCHS ICHS1 cluster was compared in scp mutants of various origins. In these mutants, sequence divergence in the cluster resulted in complete or partial loss of GmIRCHS in at least the pseudoCHS3 region. This result is consistent with the notion that the IR of pseudoCHS3 is sufficient to induce CHS silencing, and further supports that GmIRCHS is the I gene.