Hand contamination with hepatitis C virus in staff looking after hepatitis C-positive hemodialysis patients
Hand contamination with hepatitis C virus in staff looking after hepatitis C-positive hemodialysis patients
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DOI：
10.1159/000013565
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发表时间：
2000-03-01
影响因子：
4.2
通讯作者：
Dela Cruz, DM
中科院分区：
文献类型：
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作者：
Alfurayh, O;Sabeel, A;Dela Cruz, DM
背景：丙型肝炎病毒（HCV）是血液透析（HD）患者发生肝炎的主要原因。输血以外的途径在血液透析患者中的HCV传播中起作用，HCV的分子研究暗示了血液透析单位中病毒的医院传播。我们在我们的血液透析单位进行了一项临床病毒学研究，以调查透析人员的手是否可以代表血液透析患者中HCV的传播方式。研究方法：透析人员每次洗手使用1升无菌水，将洗涤物收集在无菌容器中，并在收集后3 h内通过聚合酶链反应（PCR）检测HCV-RNA。护士为HCV阳性患者透析80次洗手方法：对100例HCV阴性透析患者（A组）和100例透析护士洗手液（B组）进行HCV-RNA检测，并以60例透析人员进入透析室前洗手液（C组）作为对照。HCV-RNA阳性19例A组23.75%，B组8%，（p < 0.003），2（3.3%）C组样本C组2例阳性标本均为HCV阴性透析患者的护士。这些结果表明，尽管遵守了标准预防措施，但在我们的HD单元中，一些透析人员的手上仍存在HCV-RNA。因此，透析人员的手是促进HD患者之间HCV传播的潜在模式。版权所有（C）2000 S. Karger AG，巴塞尔。
Background: Hepatitis C virus (HCV) is a major cause of hepatitis in hemodialysis (HD) patients. Routes other than blood transfusion play a role in the spread of HCV in HD patients, Molecular studies of HCV implicate nosocomial transmission of the virus in HD units. We con ducted a clinicovirological study in our HD unit to investigate if the hands of dialysis personnel could represent a mode of transmission of HCV among HD patients. Methods: One liter of sterile water was used for each handwashing of dialysis personnel, The washing was collected in a sterile container and tested for HCV-RNA by polymerase chain reaction (PCR) within 3 h of collection. Eighty handwashings from nurses dialyzing HCV-positive patients (groupe A) and 100 handwashing from nurses dialyzing HCV-negative patients (group B) were tested for HCV-RNA, As a control, 60 handwashings were collected from the dialysis personnel before entering the dialysis unit (group C) and tested for HCV-RNA, Results: HCV-RNA was positive in 19 (23.75%) of samples of group A, in 8 (8%) of samples of group B (p < 0.003) and in 2 (3.3%) of samples of group C (p < 0.35), These two positive samples of group C were from nurses who had dialyzed HCV-negative patients, Conclusion: These results indicate the presence of HCV-RNA on the hands of some dialysis personnel in our HD unit, in spite of adherence to the standard precautions. The hands of dialysis personnel are therefore a potential mode for facilitating transmission of HCV between HD patients. Copyright (C) 2000 S. Karger AG, Basel.