Novel rapid method for the characterisation of polymeric sugars from macroalgae.
Novel rapid method for the characterisation of polymeric sugars from macroalgae.
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DOI：
10.1007/s10811-016-0995-0
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发表时间：
2017
影响因子：
3.3
通讯作者：
Winters AL
中科院分区：
文献类型：
--
作者：
Spicer SE;Adams JMM;Thomas DS;Gallagher JA;Winters AL
海带多糖是仅在棕色海藻中发现的储存多糖，特别是海带和墨角藻。海带多糖已被证明具有抗细胞凋亡和抗肿瘤活性，被认为是一种可以对人类健康产生积极影响的营养保健成分。结构取决于物种，通常由具有链内 β(1-6) 支链的线性 ß(1-3) 葡聚糖组成，并根据收获季节和环境因素而变化。目前分析摩尔质量和 DP 长度的方法技术要求较高，并且尚未广泛应用。在这里，我们提出了一种简单廉价的方法，可以使用带有脉冲电流检测的高性能阴离子交换色谱法（HPAEC-PAD）快速分析来自大型藻类生物质的昆布多糖，而无需水解或进一步处理。这是基于使用 DP 范围为 2 至 8 的标准 1,3-β-d-低聚葡萄糖在 CarboPac PA-100 柱 (Dionex) 上分离昆布多糖后，log10 DP 与保留时间之间观察到的线性关系。该方法用于分析从威尔士岩石海岸潮间带内收获的不同物种提取物中的昆布多糖低聚物，其中含有不同范围的昆布多糖聚合物。 DP。聚合度和外推摩尔质量与 LC-ESI/MSn 分析估计的值以及文献中报道的值非常吻合。
Laminarins are storage polysaccharides found only in brown seaweeds, specifically Laminarialaes and Fucales. Laminarin has been shown to have anti-apoptotic and anti-tumoural activities and is considered as a nutraceutical component that can positively influence human health. The structure is species dependent, generally composed of linear ß(1–3) glucans with intrachain β(1–6) branching and varies according to harvest season and environmental factors. Current methods for analysis of molar mass and DP length are technically demanding and are not widely available. Here, we present a simple inexpensive method which enables rapid analysis of laminarins from macroalgal biomass using high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) without the need for hydrolysis or further processing. This is based on the linear relationship observed between log10 DP and retention time following separation of laminarins on a CarboPac PA-100 column (Dionex) using standard 1,3-β-d-gluco-oligosaccharides ranging in DP from 2 to 8. This method was applied to analyse laminarin oligomers in extracts from different species harvested from within the intertidal zone on Welsh rocky shores containing laminarin polymers with different ranges of DP. The degree of polymerisation and extrapolated molar mass agreed well with values estimated by LC-ESI/MSn analysis and those reported in the literature.