Diploid, tetraploid, and octoploid plants from anther culture of tetraploid orchard grass, Dactylis glomerata L.
Diploid, tetraploid, and octoploid plants from anther culture of tetraploid orchard grass, Dactylis glomerata L.
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DOI：
10.1111/j.1439-0523.1997.tb00994.x
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发表时间：
1997-07-01
期刊：
影响因子：
2
通讯作者：
Andersen, SB
中科院分区：
文献类型：
--
作者：
Christensen, JR;Borrino, E;Andersen, SB
本文报道了鸭茅雄核发育的研究，主要目的是寻找花药培养反应的无性系。比较了两种培养基和两种糖对花药培养诱导和成苗的效果，用R2 M和FW两种不同的培养基对108个克隆基因型中的28个进行了花药培养，获得了胚状体。两种诱导培养基都支持培养的花药形成胚，但胚的形成量是对照的4.5倍左右。R2 M处理每100个花药获得胚数为0.81个，获得的绿色植株数是FW的5.5倍（0.054绿色植株/100花药）。在碳水化合物来源的研究中，用麦芽糖代替R2 M中的蔗糖，测试来自基因型研究的应答克隆。用麦芽糖代替蔗糖增加了胚的形成，使每100个花药中获得133个胚，而用蔗糖获得的每100个花药中获得7.1个胚。与蔗糖相比，麦芽糖也提高了获得的绿色植物的总数，分别导致每100个培养的花药66.3和1.9个绿色植物。
This report describes a study of androgenesis in Dactylis glomerata, where the main aim was to find anther culture-responsive clones. Two types of media and two sugars were compared for their effectiveness in anther culture induction and subsequent plantlet production.Embryo formation from the cultured anthers was obtained from 28 of the 108 cloned genotypes using two different substrates, R2M and FW. Both induction media supported the formation of embryos from the cultured anthers, but around 4.5 times more embryos (0.81 embryos per 100 anthers) were obtained with R2M compared with FW, and R2M also gave 5.5 times more green plants (0.054 green plants per 100 anthers) than the FW substrate.In the investigation of a carbohydrate source, responsive clones from the genotype study were tested using maltose as a substitute for sucrose in R2M. Using maltose instead of sucrose increased embryo formation so that 133 embryos per 100 anthers were obtained compared with 7.1 embryos per 100 anthers obtained with sucrose. The total number of green plants obtained was also improved with maltose compared with sucrose, resulting in 66.3 and 1.9 green plants per 100 cultured anthers, respectively.