DO ALL PROGRAMMED CELL DEATHS OCCUR VIA APOPTOSIS
DO ALL PROGRAMMED CELL DEATHS OCCUR VIA APOPTOSIS
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DOI：
10.1073/pnas.90.3.980
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发表时间：
1993-02-01
影响因子：
11.1
通讯作者：
OSBORNE, BA
中科院分区：
文献类型：
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作者：
SCHWARTZ, LM;SMITH, SW;OSBORNE, BA
在发育过程中，大量细胞通过一种称为程序性细胞死亡的非病理过程死亡。在许多组织中，死亡的细胞在形态和染色体DNA结构上表现出相似的变化，这被称为细胞凋亡。细胞凋亡是一种被广泛记录的现象，许多作者认为所有程序性细胞死亡都是通过这一过程发生的。两个典型的细胞程序性死亡模型系统是：(I)小鼠胸腺T细胞在负选择过程中的死亡；(Ii)六纹夜蛾在变态末期节间肌肉的丧失。在这份报告中，我们比较了T细胞和节间肌肉和皮肤的细胞死亡模式，它们在细胞表面形态、核超微结构、DNA片段化和多泛素基因表达方面存在差异。与T细胞不同的是，我们发现节间肌肉几乎没有表现出细胞凋亡的特征。这些数据表明，在发育过程中使用了不止一种细胞死亡机制。
During development, large numbers of cells die by a nonpathological process referred to as programmed cell death. In many tissues, dying cells display similar changes in morphology and chromosomal DNA organization, which has been termed apoptosis. Apoptosis is such a widely documented phenomenon that many authors have assumed all programmed cell deaths occur by this process. Two well-characterized model systems for programmed cell death are (i) the death of T cells during negative selection in the mouse thymus and (ii) the loss of intersegmental muscles of the moth Manduca sexta at the end of metamorphosis. In this report we compare the patterns of cell death displayed by T cells and the intersegmental muscles and rind that they differ in terms of cell-surface morphology, nuclear ultrastructure, DNA fragmentation, and polyubiquitin gene expression. Unlike the T cells, which are known to die via apoptosis, we find that the intersegmental muscles display few of the features that characterize apoptosis. These data suggest that more than one cell death mechanism is used during development.