Taurine inhibits oxidative damage and prevents fibrosis in carbon tetrachloride-induced hepatic fibrosis
Taurine inhibits oxidative damage and prevents fibrosis in carbon tetrachloride-induced hepatic fibrosis
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DOI：
10.1016/j.jhep.2005.01.033
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发表时间：
2005-07-01
影响因子：
25.7
通讯作者：
Bouscarel, B
中科院分区：
文献类型：
--
作者：
Miyazaki, T;Karube, M;Bouscarel, B
背景/目标：方法：肝损伤组（HD组）给予四氯化碳（CCl 4）5周，另一组给予含2%牛磺酸的饲料6周（HDT）。结果：HD组大鼠肝内牛磺酸含量明显降低;牛磺酸治疗后，这种损失和肝组织学损伤和纤维化（特别是在中央周围区域）减少。此外，HD组血清和肝脏中的肝脏α-SMA、脂质过氧化氢和8-OHdG水平以及肝脏TGF-β（1）mRNA和羟脯氨酸水平显著升高，牛磺酸治疗后这些参数中的大多数显著降低。与MAP-激酶和Akt的表达，这保持不变，脂质过氧化氢和羟脯氨酸的浓度，以及TGF-β（1）mRNA水平显着降低牛磺酸在活化的HSC.Conclusions：口服牛磺酸管理增强肝牛磺酸积累，减少氧化应激，防止肝纤维化的进展，在四氯化碳诱导的HD大鼠，以及抑制HSC的转化。(c)2005年欧洲肝脏研究协会。出版社：Elsevier 13 N All rights reserved.
Background/Aims: The aim of the study was to examine the effects of taurine on hepatic fibrogenesis and in isolated hepatic stellate cells (HSC).Methods: The rats of the hepatic damage (HD) group were administered carbon tetracholoride (CCl4) for 5 weeks and a subgroup received, in addition, a 2% taurine containing diet for 6 weeks (HDT). The HSC were isolated from normal rats and cultured for 4 days.Results: The hepatic taurine concentration was decreased in the HD group. This loss and the hepatic histological damage and fibrosis (particularly in the pericentral region), were reduced following taurine treatment. Furthermore, the hepatic alpha-SMA, lipid hydroperoxide and 8-OHdG levels in serum and liver, as well as hepatic TGF-beta(1) mRNA and hydroxyproline levels were significantly increased in the HD group, and most of these parameters were significantly reduced following taurine treatment. In contrast to the MAP-kinase and Akt expressions, which remained unchanged, the lipid hydroperoxide and hydroxyproline concentrations, as well as TGF-beta(1) mRNA levels were significantly reduced by taurine in activated HSC.Conclusions: Oral taurine administration enhances hepatic taurine accumulation, reduces oxidative stress and prevents progression of hepatic fibrosis in CCl4-induced HD rats, as well as inhibits transformation of the HSC. (c) 2005 European Association for the Study of the Liver. Published by Elsevier 13N. All rights reserved.