CIRCULATING COMPLEXES CONTAINING IGA AND FIBRONECTIN IN PATIENTS WITH PRIMARY IGA NEPHROPATHY
CIRCULATING COMPLEXES CONTAINING IGA AND FIBRONECTIN IN PATIENTS WITH PRIMARY IGA NEPHROPATHY
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DOI：
10.1073/pnas.85.13.4865
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发表时间：
1988-07-01
影响因子：
11.1
通讯作者：
HEINEGARD, D
中科院分区：
文献类型：
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作者：
CEDERHOLM, B;WIESLANDER, J;HEINEGARD, D
来自原发性伊加肾病患者的伊加抗体与胶原蛋白I、II和IV结合。在这里，我们表明，这种结合是介导的纤维连接蛋白的胶原结合位点，发生在循环中的伊加复合物。未鉴定出与胶原蛋白直接结合的抗体。通过明胶-琼脂糖凝胶和肝素-琼脂糖凝胶上的亲和色谱分离复合物，两者都具有对纤连蛋白的亲和力，然后吸附到固定在琼脂糖凝胶上的抗人伊加。纤连蛋白和伊加抗体在分离的复合物中的存在下，通过酶联免疫吸附试验，凝胶电泳，电泳转移，然后免疫染色。血清中IgA-纤连蛋白复合物的存在以及该复合物与胶原的结合证明了在鉴定抗胶原抗体之前从血清中去除纤连蛋白的必要性。
IgA antibodies from patients with primary IgA nephropathy bind to collagens I, II, and IV. Here we show that this binding is mediated by the collagen-binding site of fibronectin, which occurs in the circulation in complex with IgA. No antibodies binding directly to collagen were identified. The complexes were isolated by affinity chromatography on gelatin-Sepharose and heparin-Sepharose, both with affinity for fibronectin, followed by adsorption to anti-human IgA immobilized on agarose gel. The presence of fibronectin and IgA antibodies in the isolated complexes is shown by enzyme-linked immunosorbent assay, gel electrophoresis, and electrophoretic transfer followed by immunostaining. The presence of an IgA-fibronectin complex in serum and the binding of this complex to collagen demonstrate the necessity of removing fibronectin from serum prior to identifying anti-collagen antibodies.