Messenger RNA Sequencing and Pathway Analysis Provide Novel Insights Into the Susceptibility to Salmonella enteritidis Infection in Chickens.
Messenger RNA Sequencing and Pathway Analysis Provide Novel Insights Into the Susceptibility to Salmonella enteritidis Infection in Chickens.
复制标题
Messenger RNA测序和途径分析提供了对鸡在鸡肉肠道肠道内感染的易感性的新见解。
DOI：
10.3389/fgene.2018.00256
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发表时间：
2018
影响因子：
3.7
通讯作者：
Wen J
中科院分区：
文献类型：
--
作者：
Li P;Fan W;Everaert N;Liu R;Li Q;Zheng M;Cui H;Zhao G;Wen J
肠炎沙门氏菌(Salmonella Enteritidis，SE)是一种对动物和人类健康都有负面影响的食源性病原体。家禽被认为是疾病的重要来源和携带者，控制家禽SE感染对人类公共卫生具有重要的现实意义。为了确定免疫相关的基因标记和免疫途径，本研究对攻击敏感(S)、攻击抵抗(R)和非攻击(C)雏鸡(3日龄，无特定病原体的白来航鸡)的脾转录进行了分析。通过比较，共鉴定出934个显著差异表达基因(DEG)。本文首次报道了Forkhead box O(FOXO)信号通路中涉及的DIGs，特别是FOXO_3，被鉴定为寄主抵抗SE侵染的潜在标记。攻击易感鸡表现出FoxO信号通路的强烈激活，这可能是SE诱导的病理过程中导致免疫细胞凋亡的一个主要缺陷；这些S鸡还表现出丝裂原活化蛋白激酶相关基因的弱激活，而R鸡表现出强烈的脾激活。有趣的是，在对沙门氏菌的免疫反应中，有几个途径受到抑制，包括细胞因子-细胞因子受体相互作用和JAK-STAT，只有在S禽类中才发现这些途径之间存在串扰，这可能是导致沙门氏菌感染的原因之一。这些发现将有助于通过沙门氏菌诱导的途径在宿主免疫反应的早期阶段了解对SE感染的抵抗力和易感性，为制定SE预防和治疗策略提供新的途径，并可能通过遗传选择来增强动物的先天抵抗力。
Salmonella enteritidis (SE) is a foodborne pathogen that negatively affects both animal and human health. Controlling poultry SE infection will have great practical significance for human public health, as poultry are considered to be important sources and carriers of the disease. In this study, the splenic transcriptomes of challenged-susceptible (S), challenged-resistant (R) and non-challenged (C) chicks (3-days old, specific-pathogen-free White Leghorn) were characterized in order to identify the immune-related gene markers and pathways. A total of 934 significant differentially expressed genes (DEGs) were identified in comparisons among the C, R and S birds. First reported here, the DEGs involved in the Forkhead box O (FoxO) signaling pathway, especially FoxO3, were identified as potential markers for host resistance to SE infection. The challenged-susceptible birds exhibited strong activation of the FoxO signaling pathway, which may be a major defect causing immune cell apoptosis as part of SE-induced pathology; these S birds also showed weak activation of mitogen-activated protein kinase (MAPK)-related genes, contrasting with strong splenic activation in the R birds. Interestingly, suppression of several pathways in the immune response against Salmonella, including cytokine-cytokine receptor interaction and Jak-STAT, was only found in S birds and there was evidence of cross-talk among these pathways, perhaps contributing to susceptibility to Salmonella infection. These findings will help facilitate understanding resistance and susceptibility to SE infection in the earliest phases of the host immune response through Salmonella-induced pathways, provide new approaches to develop strategies for SE prevention and treatment, and may enhance innate resistance by genetic selection in animals.