Molecular variation in the nucleoprotein gene (ORF7) of the porcine reproductive and respiratory syndrome virus (PRRSV)
Molecular variation in the nucleoprotein gene (ORF7) of the porcine reproductive and respiratory syndrome virus (PRRSV)
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DOI：
10.1016/s0168-1702(97)00146-9
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发表时间：
1998-03-01
期刊：
影响因子：
5
通讯作者：
Jestin, A
中科院分区：
文献类型：
--
作者：
Le Gall, A;Legeay, O;Jestin, A
对 15 个欧洲猪繁殖与呼吸综合征病毒 (PRRSV) 分离株的核蛋白基因 (ORF7) 进行了测序，并与其他 PRRSV 分离株（2 个欧洲和 13 个美洲）以及其他动脉病毒各一个分离株（乳酸脱氢酶升高病毒 (LDV)、猿出血热病毒 (SHFV) 和马动脉炎病毒 (EAV)）的相应序列进行了比较。他们的系统发育关系是使用邻接和简约方法建立的。区分了四个谱系（PRRSV、LDV、SHFV 和 EAV）。可以进一步鉴定PRRSV的两种基因型，即欧洲型和美洲型。 PRRSV 的欧洲基因型高度保守。对 PRRSV ORF7 中核苷酸和氨基酸取代的分析揭示了四个稳定区域，可能是由于它们对核衣壳功能和/或结构的需要而保守的。当研究同义突变或非同义突变时，无法精确确定 ORF7 中恒定突变的积累。欧洲PRRSV在体内的传代对ORF7序列几乎没有影响：仅检测到ORF7中的少量同义取代，证实了其低变异性。 (C) 1998 Elsevier Science B.V. 保留所有权利。
The nucleoprotein gene (ORF7) of 15 European isolates of porcine reproductive and respiratory syndrome virus (PRRSV) was sequenced and compared with corresponding sequences of other PRRSV isolates (2 European and 13 American) and one isolate each of other arteriviruses (the lactate dehydrogenase elevating virus (LDV), the simian haemorrhagic fever virus (SHFV) and the equine arteritis virus (EAV)). Their phylogenetic relationships were established using neighbour-joining and parsimony methods. Four lineages (PRRSV, LDV, SHFV and EAV) were discriminated. Two genotypes of PRRSV, European and American, could be further identified. The European genotype of PRRSV was highly conserved. Analysis of the nucleotide and amino acid substitutions in PRRSV ORF7 revealed four stable regions, probably conserved because of their requirement for nucleocapsid function and/or structure. No constant mutations accumulation in the ORF7 could be determined precisely when either synonymous or non-synonymous mutations were studied. Passage of the European PRRSV in vivo had little in influence on the ORF7 sequence: only a small number of synonymous substitutions in ORF7 was detectable, confirming its low variability. (C) 1998 Elsevier Science B.V. All rights reserved.