A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice
A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice
复制标题
有害的线粒体-核相互作用导致水稻细胞质雄性不育
DOI：
10.1038/ng.2570
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发表时间：
2013-05-01
期刊：
影响因子：
30.8
通讯作者：
Liu, Yao-Guang
中科院分区：
文献类型：
--
作者：
Luo, Dangping;Xu, Hong;Liu, Yao-Guang
植物细胞质雄性不育（CMS）是由于细胞器和细胞核基因组之间的不相容性而导致的，它阻止了自花授粉，使杂交作物育种能够提高产量。自20世纪70年代以来，野败型CMS（CMS-WA）已被用于大多数“三系”杂交水稻生产中，但该性状的分子基础仍不清楚。在这里，我们报告了一个新的线粒体基因，WA 352，最近起源于野生稻，赋予CMS-WA，因为它编码的蛋白质与核编码的线粒体蛋白COX 11相互作用。在CMS-WA系中，WA 352优先在花药绒毡层中积累，从而抑制COX 11在过氧化物代谢中的功能，并引发绒毡层细胞程序性死亡和随后的花粉败育。WA 352诱导的不育性可以被两个育性恢复基因（Rf）抑制，这表明存在不同的机制来抵消有害的细胞质因子。因此，CMS相关的细胞质-核不相容性是由新进化的线粒体基因和保守的必需核基因之间的有害相互作用驱动的。
Plant cytoplasmic male sterility (CMS) results from incompatibilities between the organellar and nuclear genomes and prevents self pollination, enabling hybrid crop breeding to increase yields,,,,,. The Wild Abortive CMS (CMS-WA) has been exploited in the majority of 'three-line' hybrid rice production since the 1970s, but the molecular basis of this trait remains unknown. Here we report that a new mitochondrial gene,WA352, which originated recently in wild rice, confers CMS-WA because the protein it encodes interacts with the nuclear-encoded mitochondrial protein COX11. In CMS-WA lines, WA352 accumulates preferentially in the anther tapetum, thereby inhibiting COX11 function in peroxide metabolism and triggering premature tapetal programmed cell death and consequent pollen abortion. WA352-induced sterility can be suppressed by two restorer-of-fertility (Rf) genes, suggesting the existence of different mechanisms to counteract deleterious cytoplasmic factors. Thus, CMS-related cytoplasmic-nuclear incompatibility is driven by a detrimental interaction between a newly evolved mitochondrial gene and a conserved, essential nuclear gene.