Inhibitory effects of glycolipids fraction from spinach on mammalian DNA polymerase activity and human cancer cell proliferation
Inhibitory effects of glycolipids fraction from spinach on mammalian DNA polymerase activity and human cancer cell proliferation
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DOI：
10.1016/j.jnutbio.2005.02.007
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发表时间：
2005-10-01
影响因子：
5.6
通讯作者：
Mizushina, Y
中科院分区：
文献类型：
--
作者：
Kuriyama, I;Musumi, K;Mizushina, Y
我们成功地从干蔬菜中纯化出单半乳糖二酰甘油、二半乳糖二酰甘油和磺基喹诺酮二酰甘油 (SQDG) 中含有主要糖脂的级分。这种糖脂级分在体外是 DNA 聚合酶 α (pol α) 的抑制剂，也是人类癌细胞增殖的抑制剂。本研究对八种常见蔬菜的糖脂成分、主要糖脂含量、哺乳动物DNA聚合酶抑制活性和对人类癌细胞的抗增殖活性进行了研究。绿茶总体上含有最多的糖脂。菠菜中SQDG含量最高，其次是欧芹、大葱、细香葱、甜椒、绿茶、胡萝卜和大蒜。菠菜对pol alpha活性和人类癌细胞增殖具有最强的抑制作用。 SQDG 含量与 DNA 聚合酶抑制之间存在显着相关性。因此，SQDG对pol a活性的抑制可能导致细胞生长抑制。在测试的6个菠菜亚种中，“安娜”的SQDG含量最高，对DNA聚合酶的抑制活性最强，对人类癌细胞增殖的作用最大。基于这些结果，菠菜中的糖脂部分可能是具有新型抗癌活性的食品材料来源。 (c) 2005 Elsevier Inc. 保留所有权利。
We succeeded in purifying the fraction containing the major glycolipids in monogalactosyl diacylglycerol, digalactosyl diacylglycerol and sulfoquinovosyl diacylglycerol (SQDG) from dried vegetables. This glycolipids fraction was an inhibitor of DNA polymerase alpha (pol alpha) in vitro and also the proliferation of human cancer cells. In this study, eight common vegetables were investigated in terms of the glycolipids fraction, the amounts of major glycolipids, mammalian DNA polymerase inhibitory activity and antiproliferative activity toward human cancer cells. Green tea possessed the largest amount of glycolipids overall. Spinach contained the largest amount of SQDG, followed by parsley, green onion, chive, sweet pepper, green tea, carrot and garlic. Spinach had the strongest inhibitory effect on pol alpha activity and human cancer cell proliferation. A significant correlation was found between SQDG content and inhibition of DNA polymerase. Therefore, the inhibition of pol a activity by SQDG may lead to cell growth suppression. Of the six subspecies of spinach (Spinacia oleracea) tested, "Anna" had the largest amount of SQDG, strongest inhibitory activity toward DNA polymerase and greatest effect on human cancer cell proliferation. Based on these results, the glycolipids fraction from spinach is potentially a source of food material for a novel anticancer activity. (c) 2005 Elsevier Inc. All rights reserved.