Mn(II) tags for DEER distance measurements in proteins via C-S attachment
Mn(II) tags for DEER distance measurements in proteins via C-S attachment
复制标题
Mn(II) 标签，用于通过 C-S 连接测量蛋白质中的 DEER 距离。
DOI：
10.1039/c5dt04123f
复制
发表时间：
2015-01-01
影响因子：
4
通讯作者：
Goldfarb, Daniella
中科院分区：
文献类型：
--
作者：
Martorana, Andrea;Yang, Yin;Goldfarb, Daniella
研制了用于脉冲EPR光谱测量Mn2+-Mn2+距离的Mn2+螯合标记物。它们具有与蛋白质稳定的C-S偶联，对半胱氨酸硫醇的高反应性，以及可用于还原条件下高分辨率距离测量的短而硬的连接体。用这些标签标记的泛素在95 GHz处进行了双电子-电子共振测量，得到了预期的窄距离分布。
Mn2+ chelating tags for Mn2+-Mn2+ distance measurements by pulse EPR spectroscopy were developed. They feature a stable C-S conjugation to the protein, high reactivity towards cysteine thiols and short and rigid linkers that can be used in distance measurements with high resolution under reductive conditions. Double electron-electron resonance measurements at 95 GHz on ubiquitin labeled with these tags showed the expected narrow distance distribution.