Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles
Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles
复制标题
基于多色量子点和金纳米粒子的多重电化学发光DNA传感器测定乙型肝炎病毒和丙型肝炎病毒
DOI：
10.1016/j.aca.2016.02.024
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发表时间：
2016-04-15
影响因子：
6.2
通讯作者：
Su, Xingguang
中科院分区：
文献类型：
--
作者：
Liu, Linlin;Wang, Xinyan;Su, Xingguang
本文基于碲化镉量子点（CdTe QDs）和金纳米粒子（Au NPs），构建了一种新型的多重电化学发光（ECL）DNA传感器，用于检测B肝炎病毒（HBV）和丙型肝炎病毒（HCV）。选择电化学合成的石墨烯纳米片（GNs）作为导电桥将锚量子点（551）-捕获DNA（HBV）和CdTe量子点（607）-捕获DNA（HCV）锚定在玻碳电极（GCE）上。然后，引入不同浓度的靶DNA（HBV）和靶DNA（HCV）与互补的CdTe量子点捕获DNA杂交。将Au NPs-探针DNA（HBV）和Au NPs-探针DNA（HCV）通过与未反应的互补CdTe QDs-捕获DNA杂交而修饰到上述复合膜上。Au NPs的内过滤效应使CdTe量子点的电致化学发光（ECL）发生猝灭。因此，基于Au纳米粒子-探针DNA/靶DNA/CdTe量子点-捕获DNA/GNs/GCE复合膜的ECL DNA传感器可以实现对靶DNA（HBV）和靶DNA（HCV）的检测。在最佳条件下，CdTe量子点（551）和CdTe量子点（607）的电化学发光强度分别与靶DNA（HBV）和靶DNA（HCV）的浓度在0.0005-0.5 nmol L-1和0.001 - 1.0 nmol L-1范围内呈良好的线性关系，检出限分别为0.082 pmol L-1和0.34 pmol L-1（S/N = 3）。该传感器具有良好的灵敏度、选择性、重现性和稳定性。将该传感器用于人血清样品中靶DNA（HBV）和靶DNA（HCV）的测定，结果令人满意。(C)© 2016 Elsevier B. V.版权所有。
In this work, a novel multiplex electrochemiluminescence (ECL) DNA sensor has been developed for determination of hepatitis B virus (HBV) and hepatitis C virus (HCV) based on multicolor CdTe quantum dots (CdTe QDs) and Au nanoparticles (Au NPs). The electrochemically synthesized graphene nanosheets (GNs) were selected as conducting bridge to anchor CdTe QDs(551)-capture DNA(HBV) and CdTe QDs(607)-capture DNA(HCV) on the glassy carbon electrode (GCE). Then, different concentrations of target DNA(HBV) and target DNA(HCV) were introduced to hybrid with complementary CdTe QDs-capture DNA. Au NPs-probe DNA(HBV) and Au NPs-probe DNA(HCV) were modified to the above composite film via hybrid with the unreacted complementary CdTe QDs-capture DNA. Au NPs could quench the electrochemiluminescence (ECL) intensity of CdTe QDs due to the inner filter effect. Therefore, the determination of target DNA(HBV) and target DNA(HCV) could be achieved by monitoring the ECL DNA sensor based on Au NPs-probe DNA/target DNA/CdTe QDs-capture DNA/GNs/GCE composite film. Under the optimum conditions, the ECL intensity of CdTe QDs(551) and CdTe QDs(607) and the concentration of target DNA(HBV) and target DNA(HCV) have good linear relationship in the range of 0.0005-0.5 nmol L-1 and 0.001 -1.0 nmol L-1 respectively, and the limit of detection were 0.082 pmol L-1 and 0.34 pmol L-1 respectively (S/N = 3). The DNA sensor showed good sensitivity, selectivity, reproducibility and acceptable stability. The proposed DNA sensor has been employed for the determination of target DNA(HBV) and target DNA(HCV) in human serum samples with satisfactory results. (C) 2016 Elsevier B.V. All rights reserved.