LONG-TERM IN VIVO REGENERATION OF PERIPHERAL NERVES THROUGH BIOENGINEERED NERVE GRAFTS
LONG-TERM IN VIVO REGENERATION OF PERIPHERAL NERVES THROUGH BIOENGINEERED NERVE GRAFTS
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DOI：
10.1016/j.neuroscience.2011.02.052
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发表时间：
2011-05-05
期刊：
影响因子：
3.3
通讯作者：
Terenghi, G.
中科院分区：
文献类型：
--
作者：
di Summa, P. G.;Kalbermatten, D. F.;Terenghi, G.
尽管自体神经移植仍然是神经重建的首选策略，但它具有牺牲功能神经的严重缺点。生物人工导管中的细胞移植是改善神经再生的另一种策略。神经纤维蛋白导管中接种了多种细胞类型：原代施万细胞（SC）、SC样分化的骨髓源性间充质干细胞（dMSC）、SC样分化的脂肪源性干细胞（dASC）。另外两个对照组是没有细胞和自体移植物的纤维蛋白导管。在一项长期实验（16 周）中，使用导管桥接 1 厘米的大鼠坐骨神经间隙。研究了再生神经的功能和形态特性。与空纤维蛋白导管相比，在自体移植物和所有细胞接种组中观察到肌肉萎缩减少。 SC 显示再生神经的轴突髓鞘形成和平均纤维直径显着改善。就轴突和纤维直径的改善、腓肠肌水平的诱发电位以及运动神经元的再生而言，dASC 是最有效的细胞群，与自体移植物相似。鉴于这些结果以及脂肪干细胞的其他优点（例如易于收获和相对丰度），dASC 可能是一种临床上可转化的途径，可实现增强周围神经修复的新方法。 (C) 2011 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Although autologous nerve graft is still the first choice strategy in nerve reconstruction, it has the severe disadvantage of the sacrifice of a functional nerve. Cell transplantation in a bioartificial conduit is an alternative strategy to improve nerve regeneration. Nerve fibrin conduits were seeded with various cell types: primary Schwann cells (SC), SC-like differentiated bone marrow-derived mesenchymal stem cells (dMSC), SC-like differentiated adipose-derived stem cells (dASC). Two further control groups were fibrin conduits without cells and autografts. Conduits were used to bridge a 1 cm rat sciatic nerve gap in a long term experiment (16 weeks). Functional and morphological properties of regenerated nerves were investigated. A reduction in muscle atrophy was observed in the autograft and in all cell-seeded groups, when compared with the empty fibrin conduits. SC showed significant improvement in axon myelination and average fiber diameter of the regenerated nerves. dASC were the most effective cell population in terms of improvement of axonal and fiber diameter, evoked potentials at the level of the gastrocnemius muscle and regeneration of motoneurons, similar to the autografts. Given these results and other advantages of adipose derived stem cells such as ease of harvest and relative abundance, dASC could be a clinically translatable route towards new methods to enhance peripheral nerve repair. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.