Drosophila TRPA1 functions in temperature control of circadian rhythm in pacemaker neurons.
Drosophila TRPA1 functions in temperature control of circadian rhythm in pacemaker neurons.
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DOI：
10.1523/jneurosci.4237-12.2013
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发表时间：
2013-04-17
期刊：
影响因子：
--
通讯作者：
Montell C
中科院分区：
文献类型：
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作者：
Lee Y;Montell C
从苍蝇到人类，大多数动物都依靠生物钟来同步它们的生理和行为。日常的光循环是众所周知的设定昼夜节律的环境线索。模拟白天和黑夜的温暖和凉爽的温度对大多数动物的昼夜活动也是有效的。即使脊椎动物也可以通过暴露在温度循环中而表现出昼夜节律反应。在像果蝇这样的变温动物中，只有2-3摄氏度的温差就足以使运动节奏同步。然而，参与果蝇或其他动物昼夜活动温度调节的分子传感器是个谜。目前也不清楚这种探测器是否仅限于外周，或者可能位于中央大脑。在这里，我们证明了果蝇TRPA1(瞬时受体电位阳离子通道A1)是温度循环中正常活动模式所必需的。TRPA1基因在中央脑中的起搏神经元亚群中表达。作为对温度夹带的反应，TRPA1的缺失削弱了起搏器神经元的活性，并改变了起搏器神经元中昼夜节律时钟蛋白周期(PER)的表达。这些发现强调了热色氨酸在温度调节中的作用，它超出了避免有害或次佳温度的范围。
Most animals from flies to humans count on circadian clocks to synchronize their physiology and behaviors. Daily light cycles are well-known environmental cues for setting circadian rhythms. Warmer and cooler temperatures that mimic day and night are also effective in entraining circadian activity in most animals. Even vertebrate organisms can be induced to show circadian responses through exposure to temperature cycles. In poikilothermic animals such as Drosophila, temperature differences of only 2–3°C are sufficient to synchronize locomotor rhythms. However, the molecular sensors that participate in temperature regulation of circadian activity in fruit flies or other animals are enigmatic. It is also unclear whether such detectors are limited to the periphery or may be in the central brain. Here, we showed that Drosophila TRPA1 (Transient Receptor Potential Cation Channel A1) was necessary for normal activity patterns during temperature cycles. The trpA1 gene was expressed in a subset of pacemaker neurons in the central brain. In response to temperature entrainment, loss of trpA1 impaired activity, and altered expression of the circadian clock protein Period (Per) in a subset of pacemaker neurons. These findings underscore a role for a thermoTRP in temperature regulation that extends beyond avoidance of noxious or suboptimal temperatures.