Photocleavable linker for the patterning of bioactive molecules.
Photocleavable linker for the patterning of bioactive molecules.
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DOI：
10.1038/srep18309
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发表时间：
2015-12-16
影响因子：
4.6
通讯作者：
Spatz JP
中科院分区：
文献类型：
--
作者：
Wegner SV;Sentürk OI;Spatz JP
在这里，我们报告了使用一个多功能的光裂解硝基苄基连接器的各种各样的生物活性分子和photoreleath他们的需求。在一端，接头具有NHS基团，可以与任何胺（例如肽、蛋白质或胺接头）偶联，而在另一端，具有炔，以便方便地连接到具有叠氮官能团的材料。该接头与NTA-胺或细胞粘附肽cRGD缀合，以使得能够分别在聚乙二醇化玻璃表面上直接图案化His 6标记的蛋白质或细胞。这种方法提供了一种实用的方法来控制各种各样的生物活性分子的呈现，具有高的空间和时间分辨率。也可以控制光裂解的程度以调节生物分子密度和细胞附着到表面的程度。
Herein, we report the use of a versatile photocleavable nitrobenzyl linker to micropattern a wide variety of bioactive molecules and photorelease them on demand. On one end, the linker has an NHS group that can be coupled with any amine, such as peptides, proteins or amine-linkers, and on the other end an alkyne for convenient attachment to materials with an azide functional group. This linker was conjugated with NTA-amine or the cell adhesion peptide cRGD to enable straightforward patterning of His6-tagged proteins or cells, respectively, on PEGylated glass surfaces. This approach provides a practical way to control the presentation of a wide variety of bioactive molecules with high spatial and temporal resolution. The extent of photocleavage can also be controlled to tune the biomolecule density and degree of cell attachment to the surface.