Immunoreactivity of cerebral amyloidosis is enhanced by protein denaturation treatments
Immunoreactivity of cerebral amyloidosis is enhanced by protein denaturation treatments
复制标题
蛋白质变性治疗可增强脑淀粉样变性的免疫反应性
作者：
R. Doi;T. Kitamoto;J. Tateishi
本研究应用抗人朊蛋白抗血清和抗β/A4蛋白抗血清对3例Gerstmann-Sträussler综合征患者和3例Alzheimer病或Alzheimer型老年性痴呆患者的石蜡包埋脑切片进行了研究。与硫氰酸胍，三氯乙酸和苯酚孵育后，库鲁斑和老年斑的免疫反应性增强到与甲酸处理相同的水平。这些治疗显示小的致密淀粉样沉积物、斑块核心周围的淀粉样沉积物和弥漫性斑块。这些化学物质中的大多数改变了两种淀粉样蛋白的亲脂性。这些处理可能使淀粉样原纤维蛋白变性并破坏淀粉样原纤维的结构，从而揭示埋藏的表位。
SummaryWe investigated paraffin-embedded brain sections from three patients with Gerstmann-Sträussler syndrome and three patients with Alzheimer's disease or senile dementia of Alzheimer type using anti-human prion protein antisera and anti-β/A4 protein antisera after protein denaturation treatments. After incubation with guanidine-thiocyanate, trichloroacetate, and phenol, the immunoreactivity of kuru plaques and senile plaques was enhanced to the same level as the formic acid treatment. These treatments revealed small compact amyloid deposits, amyloid deposits surrounding the plaque cores, and diffuse plaques. Most of these chemicals changed the congophilia of both amyloids. It is possible that these treatments denature amyloid fibril proteins and break down the structure of amyloid fibrils, thus revealing buried epitopes.
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影响因子：
2.9
作者：
PRUSINER, SB;BOLTON, DC;MCKINLEY, MP
通讯作者：
MCKINLEY, MP
脑淀粉样血管病的免疫组织化学研究。
DOI：
--
发表时间：
1988
期刊：
The American journal of pathology
影响因子：
--
作者：
Vinters,HV;Pardridge,WM;Secor,DL;Ishii,N
通讯作者：
Ishii,N
链脲佐菌素糖尿病大鼠膀胱试纸的长度-张力关系。
影响因子：
3.1
作者：
Longhurst,PA;Kang,J;Wein,AJ;Levin,RM
通讯作者：
Levin,RM
DOI：
10.1089/dna.1986.5.315
发表时间：
1986-08-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
KRETZSCHMAR, HA;STOWRING, LE;DEARMOND, SJ
通讯作者：
DEARMOND, SJ