Determination of long-chain fatty acid acyl-coenzyme A compounds using liquid chromatography-electrospray ionization tandem mass spectrometry
Determination of long-chain fatty acid acyl-coenzyme A compounds using liquid chromatography-electrospray ionization tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2004.05.012
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发表时间：
2004-09-05
影响因子：
3
通讯作者：
Auriola, S
中科院分区：
文献类型：
--
作者：
Mauriala, T;Herzig, KH;Auriola, S
酰基辅酶A在细胞的脂肪和葡萄糖代谢中具有重要作用。在本研究中，我们开发了一种在线 HPLC-ESI-MSIMS 方法，用于测定大鼠肝脏样品中的长链酰基辅酶 A 化合物。使用三乙胺乙酸酯和乙腈梯度，通过 C-4 反相柱分离六种长链酰基辅酶 A（C 16:0、C 16:1、C 18:0、C18:1、C20:0 和 C20:4）。负电喷雾电离非常适合酰基 CoA 化合物，可以获得长链酰基 CoA 的出色 MS/MS 谱图。采用离子阱质谱仪的 MS/MS 方法可以同时识别和定量单个酰基辅酶 A。该方法被证明足够灵敏，可以使用 0.4-0.7 g 组织测定所有感兴趣的化合物，并在 0.1-15.0 pmol/mul 范围内进行验证。 (C) 2004 Elsevier B.V. 保留所有权利。
Acyl-CoAs have important role in fat and glucose metabolism of the cells. In this study we have developed an on-line HPLC-ESI-MSIMS method for determination of long-chain acyl-CoA compounds in rat liver samples. Six long-chain acyl-CoAs (C 16:0, C 16: 1, C 18:0, C18:1, C20:0 and C20:4) were separated with a C-4 reversed-phase column using triethylamine acetate and acetonitrile gradient. Negative electrospray ionization is very suitable for acyl-CoA compounds and excellent MS/MS spectra for long-chain acyl-CoAs can be obtained. MS/MS method with an ion trap mass spectrometer makes it possible to identify and quantitate individual acyl-CoAs simultaneously. The method proved to be sensitive enough for determination of all compounds of interest using 0.4-0.7 g of tissue and was validated in the range of 0.1-15.0 pmol/mul. (C) 2004 Elsevier B.V. All rights reserved.