Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae
Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae
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感染痢疾志贺氏菌的管病毒科噬菌体的分离、表征和基于 PCR 的分子鉴定
DOI：
10.1016/j.micpath.2019.03.037
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发表时间：
2019-06-01
影响因子：
3.8
通讯作者：
Wang, Ran
中科院分区：
文献类型：
--
作者：
Shahin, Khashayar;Bao, Hongduo;Wang, Ran
背景：志贺菌是志贺菌属的一种，是引起世界各地志贺菌病暴发的主要病原菌。抗生素消费量的增加导致了耐药性菌株的出现和传播。因此，寻找新的替代感染控制是必不可少的，其中之一是使用bacteriophages.Materials和方法：裂解噬菌体抗志贺氏菌从炼油厂废水中分离。分别用TEM和测序研究噬菌体的形态和遗传特征。结果：根据形态学和遗传学结果，将该噬菌体命名为vB-SdyS-ISF 003。PCR产物的测序结果表明，vB-SdyS-ISF 003噬菌体属于Siphoviridae科Tunavirinae亚科T1病毒属。这是第一个检测到的抗S.属于Siphoviridae科。此外，该菌的寄主范围仅限于S.紫菀基因组大小约为62 kb。vB-SdyS-ISF 003噬菌体具有许多理想的特性，包括对S.结论：vB-SdyS-ISF 003是一种新的TI病毒噬菌体，具有良好的生物防治潜力。不同条件下的紫茎泽兰。
Background: Shigella dysenteriae is one of the members of Shigella genus which was the main responsible of different Shigellosis outbreaks worldwide. The increasing consumption of antibiotics has led to the emergence and spreading of antibiotic-resistant strains. Therefore, finding new alternatives for infection control is essential, one of which is using bacteriophages.Materials and methods: Lytic bacteriophage against Shigella dysenteriae was isolated from petroleum refinery wastewater. Phage morphological and genetic characteristics were studied using TEM, and sequencing, respectively. In addition, the genome size was estimated, and phage resistance to different temperatures and pH, host range, adsorption rate, and one-step growth were investigated.Results: According to the morphology and genetic results, this phage was named vB-SdyS-ISF003. Sequencing of the PCR products revealed that the vB-SdyS-ISF003 phage belongs to the species Tlvirus, subfamily Tunavirinae of family Siphoviridae. This was the first detected bacteriophage against S. dysenteriae, which belongs to the family Siphoviridae. In addition, its host range was limited to S. dysenteriae. The genome size was about 62 kb. vB-SdyS-ISF003 phage has a number of desirable characteristics including the limited host range to S. dysenteriae, very short connection time, a relatively wide range of temperature tolerance - 20 to 50 degrees C, pH tolerance of 7-9 without significant reduction in the phage titer.Conclusion: vB-SdyS-ISF003 is a novel virulent TI virus phage and has the appropriate potential for being used in bio controlling of S. dysenteriae in different condition.