Characterization of glycine-rich RNA-binding proteins in Brassica napus under stress conditions
Characterization of glycine-rich RNA-binding proteins in Brassica napus under stress conditions
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DOI：
10.1111/j.1399-3054.2012.01628.x
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发表时间：
2012-11-01
影响因子：
6.4
通讯作者：
Kang, Hunseung
中科院分区：
文献类型：
--
作者：
Kim, Min Kyung;Jung, Hyun Ju;Kang, Hunseung
虽然富含甘氨酸的RNA结合蛋白（GRP）在胁迫适应过程中的功能作用已经在拟南芥和水稻（Oryza sativa）中得到了广泛的评价，但大多数GRP的胁迫响应作用尚未在包括油菜（Brassica napus）在内的其他植物物种中得到表征。在这里，GRP在B中的特征和应激反应表达模式。甘蓝型油菜（BnGRPs）。B的基因组。napus包含7个密切相关的BnGRP，其中N-末端区域的高度保守的RNA识别基序的氨基酸序列彼此高度相似，但C-末端富含甘氨酸的区域的序列在不同的BnGRP之间差异很大。所有BnGRPs的转录水平显着上调冷胁迫，而它们的表达显着下调脱水或高盐胁迫。在评价的7个BnGRPs中，BnGRP 1的特征在于其更详细的细胞定位和功能作用，作为一个RNA伴侣在冷应激下。冷诱导的BnGRP 1成功地补充了大肠杆菌突变体BX 04细胞在冷胁迫下的冷敏感表型，并具有DNA和RNA解链能力。BnGRP 1在拟南芥中的异位表达可加速种子萌发，增强植物在低温或冰冻胁迫下的抗冻性。总的来说，这项研究的结果支持新兴的想法，GRP是功能保守的RNA分子伴侣在不同的植物物种的冷适应过程中。
Although the functional roles of glycine-rich RNA-binding proteins (GRPs) during stress adaptation have been extensively evaluated in Arabidopsis thaliana and rice (Oryza sativa), the stress-responsive roles of a majority of GRPs have not been characterized in other plant species including rapeseed (Brassica napus). Here, the characteristic features and stress-responsive expression patterns of GRPs in B. napus (BnGRPs) were investigated. The genome of B. napus contains seven closely related BnGRPs, where the amino acid sequences of a well-conserved RNA-recognition motif at the N-terminal region are highly similar to each other but the sequences of the C-terminal glycine-rich region vary greatly among different BnGRPs. The transcript levels of all BnGRPs were markedly upregulated by cold stress, while their expression was significantly downregulated by dehydration or high salinity stress. Among the seven BnGRPs evaluated, BnGRP1 was characterized in more detail for its cellular localization and functional role as an RNA chaperone under cold stress. Cold-induced BnGRP1 successfully complemented the cold-sensitive phenotype of Escherichia coli mutant BX04 cells under cold stress, and harbored DNA- and RNA-melting abilities. Ectopic expression of BnGRP1 in Arabidopsis resulted in accelerated seed germination and enhanced freezing tolerance of the plant under cold or freezing stress. Collectively, the results of this study support the emerging idea that GRPs are functionally conserved RNA chaperones during the cold adaptation process in diverse plant species.