Energy taxis is the dominant behavior in Azospirillum brasilense
Energy taxis is the dominant behavior in Azospirillum brasilense
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DOI：
10.1128/jb.182.21.6042-6048.2000
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发表时间：
2000-11-01
影响因子：
3.2
通讯作者：
Zhulin, IB
中科院分区：
文献类型：
--
作者：
Alexandre, G;Greer, SE;Zhulin, IB
能量趋向性包括趋气性、趋光性、氧化还原趋向性、替代电子受体的趋向性和可氧化底物的趋化性。此类行为的信号源自电子传输系统。能量出租车在几种微生物物种中被证明是整体行为的一部分，但它并未出现在任何微生物物种中作为主要决定因素。在这项研究中，我们表明大多数行为反应都是通过巴西α-变形菌固氮螺菌中的这种机制进行的。首先，发现对固氮螺菌栖息地典型的大多数化学效应器的趋化性是新陈代谢依赖性的，并且需要功能性电子传输系统。其次，在巴西曲霉中发现了其他与能量相关的反应，例如趋气性、氧化还原趋向性和对替代电子受体的趋向性。最后，缺乏cbb(3)型细胞色素c氧化酶的突变体的趋化性、氧化还原趋向性和趋气性受到影响。总而言之，结果表明巴西曲霉对大多数刺激的行为反应是由电子传递系统的变化触发的。
Energy taxis encompasses aerotaxis, phototaxis, redox taxis, taxis to alternative electron accepters, and chemotaxis to oxidizable substrates. The signal for this type of behavior is originated within the electron transport system. Energy taxis was demonstrated, as a part of an overall behavior, in several microbial species, but it did not appear as the dominant determinant in any of them. In this study, we show that most behavioral responses proceed through this mechanism in the alpha-proteobacterium Azospirillum brasilense. First, chemotaxis to most chemoeffectors typical of the azospirilla habitat was found to be metabolism dependent and required a functional electron transport system. Second, other energy-related responses, such as aerotaxis, redox taxis, and taxis to alternative electron accepters, were found in A. brasilense. Finally, a mutant lacking a cytochrome c oxidase of the cbb(3) type was affected in chemotaxis, redox taxis, and aerotaxis. Altogether, the results indicate that behavioral responses to most stimuli in A. brasilense are triggered by changes in the electron transport system.