The Giant Axon of the Squid: A Simple System for Axonal Transport Studies.
The Giant Axon of the Squid: A Simple System for Axonal Transport Studies.
复制标题
鱿鱼的巨型轴突：用于轴突运输研究的简单系统。
DOI：
10.1007/978-1-0716-1990-2_1
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bearer,ElaineL
中科院分区：
文献类型：
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作者：
DeGiorgis,JosephA;Jang,Marcus;Bearer,ElaineL
鱿鱼巨轴突作为一个极好的实验系统有着悠久的历史，可以用来研究有关细胞内运输的广泛问题。在本协议中，我们描述了用于解剖轴突以保持其体外活力的方法，以及将外源材料注射到活轴突中的技术。现在，鱿鱼基因组正在出现，并且 CRISPR/cas9 系统已成功应用于敲除鱿鱼基因，巨大的轴突将在强大的实验系统的科学万神殿中恢复其地位，在该系统中解决与所有真核生物有关的生物学问题。
The squid giant axon has a long history of being a superb experimental system in which to investigate a wide range of questions concerning intracellular transport. In this protocol we describe the method used for dissecting the axon to preserve its viability in vitro, and the technique for injecting exogenous materials into the living axon. Now that the squid genome is emerging, and the CRISPR/cas9 system has been successfully applied to knock-out squid genes, the giant axon will resume its place in the scientific pantheon of powerful experimental systems in which to address biological questions pertaining to all eukaryotes.