Establishment and characterization of a continuous human chondrosarcoma cell line, ch-2879:: Comparative histologic and genetic studies with its tumor of origin
Establishment and characterization of a continuous human chondrosarcoma cell line, ch-2879:: Comparative histologic and genetic studies with its tumor of origin
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DOI：
10.1097/01.lab.0000073131.34648.ea
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发表时间：
2003-06-01
影响因子：
5
通讯作者：
Llombart-Bosch, A
中科院分区：
文献类型：
--
作者：
Gil-Benso, R;Lopez-Gines, C;Llombart-Bosch, A
软骨肉瘤是恶性软骨形成肿瘤，是第二常见的骨恶性实体瘤。这些生物学知之甚少的肿瘤在临床表现和生物学行为上差异很大。化疗和放疗通常无效。在这里，我们描述了一个新的人类软骨肉瘤细胞系命名为ch-2879的建立和特点，我们比较细胞系与其肿瘤的起源。该细胞系是从胸壁复发性3级软骨肉瘤中建立的，其特征在于生长动力学和形态学研究。免疫细胞化学和RT-PCR检测软骨特异性表型的表达。使用细胞遗传学、荧光原位杂交、流式细胞术和分子技术进行遗传表征，以分析与细胞周期控制、MDM 2、CDK 4和细胞周期蛋白D1扩增以及p53基因突变有关的基因。CH-2879细胞传代培养超过80代。它们表达波形蛋白、HNK-1、HBA-71、Ki-67、细胞周期蛋白D1、Fli-1、S-100、p21、p27和p53，而不表达细胞角蛋白、EMA、p14、p16、MDM 2、Rb和c-erb-b2抗原。细胞遗传学复发肿瘤表现为超单倍体核型，克隆数量和结构异常。唯一的结构异常是t（1;21）易位的染色体衍生物。第3代的细胞系显示出两个群体：超单倍体和完全重复的亚三倍体群体。在第18代之后，仅存在亚三倍体群体。细胞表达胶原2。原发性和复发性肿瘤的分子比较证明了在复发中由9 p21基因的缺失组成的体内分子变化，这可能是由选择过程引起的。由于其基因表达谱，包括涉及在未涂层的塑料培养皿中的软骨形成的基因的表达，该细胞系可能被证明是有用的细胞和分子研究以及软骨肉瘤的表征和治疗的研究。
Chondrosarcomas are malignant cartilage-forming tumors that represent the second most common malignant solid tumor of bone. These biologically poorly understood neoplasms vary considerably in clinical presentation and biologic behavior. Chemotherapy and radiation therapy are generally ineffective. Here we describe the establishment and characterization of a new human chondrosarcoma cell line named ch-2879, and we compare the cell line with its tumor of origin. The cell line was established from a recurrent grade 3 chondrosarcoma of the chest wall and characterized by growth kinetics and morphologic studies. Immunocytochemistry and RT-PCR were performed to examine the expression of cartilage-specific phenotypes. Genetic characterization was performed using cytogenetics, fluorescence in situ hybridization, flow cytometry, and molecular techniques for analysis of the genes implicated in cell cycle control, amplification of MDM2, CDK4, and Cyclin D1, and mutations in the p53 gene. ch-2879 cells were subcultured for more than 80 passages. They expressed vimentin, HNK-1, HBA-71, Ki-67, cyclin D1, Fli-1, S-100, p21, p27, and p53 and Were negative for cytokeratin, EMA, p14, p16, MDM2, Rb, and c-erb-b2 antigens. Cytogenetically the recurrent tumor showed a hyperhaploid karyotype with clonal numerical and structural abnormalities. The sole structural abnormality was a chromosome derivative of a t(1;21) translocation. The cell line at passage 3 showed two populations: the hyperhaploid and an exactly duplicated, hypotriploid population. After the 18th passage, only the hypotriploid population was present. The cells expressed collagen 2. Molecular comparison of the primary and recurrent tumor evidenced an in vivo molecular change consisting of a deletion of 9p21 genes in the recurrence, probably caused by a selection process. Because of its gene expression profile, including expression of genes implicated in chondrogenesis in uncoated plastic dishes, this cell line may prove useful for cellular and molecular studies as well as studies of chondrosarcoma characterization and treatment.