Methanol utilizing Desulfotomaculum species utilizes hydrogen in a methanol-fed sulfate-reducing bioreactor
Methanol utilizing Desulfotomaculum species utilizes hydrogen in a methanol-fed sulfate-reducing bioreactor
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DOI：
10.1007/s00253-006-0590-4
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发表时间：
2007-01-01
影响因子：
5
通讯作者：
Stams, Alfons J. M.
中科院分区：
文献类型：
--
作者：
Balk, Melike;Weijma, Jan;Stams, Alfons J. M.
从65 ℃、甲醇为唯一能源、硫酸盐存在下的嗜热生物反应器中分离得到一株硫酸盐还原菌WW 1。菌株WW 1在甲醇或乙酸盐上的生长在200 mg l（-1）的硫化物浓度下被抑制，而在H-2/CO2上，在500 mg l（-1）的浓度下没有明显的抑制。当菌株WW 1在相同条件下与来自同一生物反应器的利用甲醇的非硫酸盐还原菌Thermotoga multeri和Moorella mulderi共培养时，观察到高达430 mg l（-1）的生长和硫化物形成。这些结果表明，在共培养物中，电子流的主要部分是从甲醇通过H-2/CO2到硫酸盐还原为硫化物。除了甲醇、乙酸盐和氢气之外，菌株WW 1还能够使用甲酸盐、苹果酸盐、富马酸盐、丙酸盐、琥珀酸盐、丁酸盐、乙醇、丙醇、丁醇、异丁醇，同时将硫酸盐还原为硫化物。在不存在硫酸盐的情况下，菌株WW 1仅在丙酮酸盐和乳酸盐上生长。16 SrRNA分析表明，菌株WW 1与热池脱硫肠菌（Desulfotomaculum thermocisternum）和澳大利亚脱硫肠菌（Desulfotomaculum australicum）的亲缘关系最近。然而，菌株WW 1的生理特性在某些方面不同于这两个相关的细菌。
A sulfate-reducing bacterium, strain WW1, was isolated from a thermophilic bioreactor operated at 65 degrees C with methanol as sole energy source in the presence of sulfate. Growth of strain WW1 on methanol or acetate was inhibited at a sulfide concentration of 200 mg l(-1), while on H-2/CO2, no apparent inhibition occurred up to a concentration of 500 mg l(-1). When strain WW1 was co-cultured under the same conditions with the methanol-utilizing, non-sulfate-reducing bacteria, Thermotoga lettingae and Moorella mulderi, both originating from the same bioreactor, growth and sulfide formation were observed up to 430 mg l(-1). These results indicated that in the co-cultures, a major part of the electron flow was directed from methanol via H-2/CO2 to the reduction of sulfate to sulfide. Besides methanol, acetate, and hydrogen, strain WW1 was also able to use formate, malate, fumarate, propionate, succinate, butyrate, ethanol, propanol, butanol, isobutanol, with concomitant reduction of sulfate to sulfide. In the absence of sulfate, strain WW1 grew only on pyruvate and lactate. On the basis of 16S rRNA analysis, strain WW1 was most closely related to Desulfotomaculum thermocisternum and Desulfotomaculum australicum. However, physiological properties of strain WW1 differed in some aspects from those of the two related bacteria.