RETRONASAL OR INTERNASAL OLFACTION CAN MEDIATE ODOR-GUIDED BEHAVIORS IN NEWBORN MICE
RETRONASAL OR INTERNASAL OLFACTION CAN MEDIATE ODOR-GUIDED BEHAVIORS IN NEWBORN MICE
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DOI：
10.1016/0031-9384(94)90234-8
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发表时间：
1994-10-01
影响因子：
2.9
通讯作者：
ARSOV, I
中科院分区：
文献类型：
--
作者：
COPPOLA, DM;COLTRANE, JA;ARSOV, I
嗅觉剥夺的研究最常使用单侧鼻孔阻塞来实现剥夺。最近的心理物理学证据表明，急性或慢性鼻孔闭塞的成年啮齿动物的嗅觉能力几乎没有下降。在这项研究中，鼻孔阻塞加上同侧或对侧嗅球传入神经阻滞对气味引导行为的影响进行了测试。还包括接受双侧球切除术或对照操作的动物。在实验1中，嗅病变产生的球吸入出生后的第二天（P2）。记录每日体重增加（哺乳成功的可靠衡量标准）直至P21。在实验2中，嗅觉损伤包括在P2上的嗅球横断。记录每日体重，直至受试者P10。双侧球切除的动物死亡率最高，生长最慢。两个鼻孔闭塞组的生长速度均比对照组慢，但在P5或P10时无显著差异。此后，它们出现了分歧，因此在P20时，与球切除术同侧鼻孔闭塞的组与对照组相似，而对侧组与双侧球切除术组相似。在实验2中，鼻孔阻塞组的体重在手术后的第二天出现差异，对侧组的体重增长最慢。行为测试结合两个实验进行分析。在P5测试受试者找到乳头的能力，在P8测试受试者找到窝的能力，在P10测试受试者对未清洗乳头和清洗乳头的偏好。只有对侧组有一个显着的抑郁症的能力，找到乳头，而所有的病变组有一个显着的，但类似的下降，在巢的发现能力。对照组和两个鼻孔闭塞组显示出显着的偏好未清洗的乳头。这些结果表明，虽然鼻孔阻塞产生嗅觉能力的一些赤字，它不会产生完全的气味剥夺。结论：阻塞的鼻窝中的嗅上皮可能通过鼻后或鼻内途径获得刺激。
Studies of olfactory deprivation have most frequently used unilateral naris occlusion to effect deprivation. Recent psychophysical evidence suggests that adult rodents with either acute or chronic naris occlusion show little decrement in olfactory ability. In this study the effect of naris occlusion coupled with ipsilateral or contralateral olfactory bulb deafferentation on odor-guided behaviors was tested in neonatal mice. Animals that received bilateral bulbectomy or control manipulation were also included. In Experiment 1, olfactory lesions were produced by bulb aspiration on the second day after birth (P2). Daily weight gain, a reliable measure of suckling success, was recorded until P21. In Experiment 2, olfactory lesions consisted of bulb transection on P2. Daily weights were recorded until subjects were P10. Animals with bilateral bulbectomy had the highest mortality rate and slowest growth race. Both naris occlusion groups grew more slowly than controls but were not significantly different at P5 or P10. They diverged, thereafter, such that at P20 the group with naris occlusion ipsilateral to bulbectomy was similar to controls, while the contralateral group was similar to the bilateral bulbectomy group. In Experiment 2, the weights of the naris occlusion groups diverged by the day after surgery, with the contralateral group suffering the most arrested growth. Behavioral tests were combined from the two experiments for analysis. Subjects were tested at P5 for their ability to find the nipple, at P8 for their ability to find the nest, and at P10 for unwashed vs. washed nipple preferences. Only the contralateral group had a significantly depressed ability to find the nipple, while all lesion groups had a significant but similar decline in nest-finding ability. Controls and both naris occlusion groups showed significant preferences for unwashed nipples. These results suggest that while naris occlusion produces some deficits in olfactory ability it does not produce complete odor deprivation. It is concluded that the olfactory epithelium in the occluded nasal fossa may gain access to stimuli by a retronasal or internasal route.