Differential response of antioxidant enzymes in leaves of necrotic wheat hybrids and their parents
Differential response of antioxidant enzymes in leaves of necrotic wheat hybrids and their parents
复制标题
DOI：
10.1034/j.1399-3054.2001.1110306.x
复制
发表时间：
2001-03-01
影响因子：
6.4
通讯作者：
Khanna-Chopra, R
中科院分区：
文献类型：
--
作者：
Dalal, M;Khanna-Chopra, R
在早期的研究中，坏死杂交小麦（Triticum aestivum L.）的叶片显示出与脂质过氧化和膜损伤增加相关的高超氧化物含量(Khanna-Chopra et al. 1998, Biochem Biophys comm248: 712-715；在本研究中，我们研究了坏死小麦杂交种Kalyansona x C306 （K x C）和WL711 x C306 （WL x C）及其亲本在不同发育阶段叶片中抗氧化酶的活性。杂交后代K × C表现出比WL × C更严重的坏死。在K × C中，超氧化物歧化酶（SOD）活性没有比亲本增加，而WL × C表现出较早的增加，但可能不足以清除增加的超氧化物，愈创木酚过氧化物酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶活性增强，过氧化氢酶活性降低，两种杂交后代均表现出明显的坏死。杂种及其亲本抗氧化酶的同工酶谱相似。愈创木酚过氧化物酶的一种同种异构体在杂交中早期出现，并随着坏死的进展而增强。结果表明，与亲本相比，坏死小麦杂种抗氧化酶的反应存在差异。在叶片发育阶段，这种反应的强度不同，这可能与杂种表达的坏死强度有关。
The leaves of necrotic hybrid of wheat (Triticum aestivum L.) exhibited high superoxide content associated with increased lipid peroxidation and membrane damage in earlier studies (Khanna-Chopra et al. 1998, Biochem Biophys Res Commun 248: 712-715; Dalal and Khanna-Chopra 1999, Biochem Biophys Res Commun 262: 109-112), In the present study, we investigated the activities of the antioxidant enzymes in the leaves of necrotic wheat hybrids, Kalyansona x C306 (K x C) and WL711 x C306 (WL x C) and their parents at different developmental stages. The K x C hybrid exhibited more severe necrosis than WL x C. In K x C, superoxide dismutase (SOD) activity showed no increase over the parents, while WL x C showed an early increase, but it was possibly insufficient to scavenge increased superoxide, Activities of guaiacol peroxidase, ascorbate peroxidase and glutathione reductase were enhanced, while catalase exhibited a decrease in activity, with the appearance of visible necrosis in both the hybrids. The isozyme profile of the antioxidant enzymes was similar in the hybrids and their parents. One existing isoform of guaiacol peroxidase showed an early appearance in the hybrid and increased in intensity with the progression of necrosis. The results reveal a differential response of antioxidant enzymes in necrotic wheat hybrids as compared to their parents. The response differed in magnitude at developmental stages of the leaves, which might be related to the intensity of necrosis expressed by the hybrids.