Identification of Immunologically Relevant Proteins of Chlamydophila abortus Using Sera from Experimentally Infected Pregnant Ewes
Identification of Immunologically Relevant Proteins of Chlamydophila abortus Using Sera from Experimentally Infected Pregnant Ewes
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DOI：
10.1128/cvi.00163-10
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发表时间：
2010-08-01
影响因子：
--
通讯作者：
Nally, J. E.
中科院分区：
文献类型：
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作者：
Marques, P. X.;Souda, Puneet;Nally, J. E.
流产嗜衣原体是一种胞内病原菌，是引起绵羊地方性流产（EAE）的病原体。C.流产病毒具有双相发育周期;细胞外感染性原体（EB）附着并穿透宿主细胞，在那里它们产生细胞内代谢活性网状体（RB）。RB通过二分裂分裂，随后成熟为EB，在感染细胞破裂时，EB被释放以感染新的宿主细胞。用2 × 10（6）个包涵体形成单位（IFU）的C对妊娠母羊进行攻毒。在卵黄囊中培养的流产牛（包括EB和RB两者）。分别于感染后0、7、14、21、27、30、35、40、43天采集血清，进行抗原鉴定。流产表现在疾病期间。此外，在30、35、40和43 dpi采集胎羊血清。从实验感染的妊娠母羊收集的所有血清样品与EB的几种抗原特异性反应，通过一维（1-D）和2-D凝胶电泳确定;通过质谱鉴定的反应性抗原包括主要外膜蛋白（MOMP），多态性外膜蛋白（POMP）和巨噬细胞感染增强因子（MIP）脂蛋白。
Chlamydophila abortus is an intracellular pathogen and the etiological agent of enzootic abortion of ewes (EAE). C. abortus has a biphasic development cycle; extracellular infectious elementary bodies (EB) attach and penetrate host cells, where they give rise to intracellular, metabolically active reticulate bodies (RB). RB divide by binary fission and subsequently mature to EB, which, on rupture of infected cells, are released to infect new host cells. Pregnant ewes were challenged with 2 x 10(6) inclusion forming units (IFU) of C. abortus cultured in yolk sac (comprising both EB and RB). Serum samples were collected at 0, 7, 14, 21, 27, 30, 35, 40, and 43 days postinfection (dpi) and used to identify antigens of C. abortus expressed during disease. Additionally, sera from fetal lambs were collected at 30, 35, 40, and 43 dpi. All serum samples collected from experimentally infected pregnant ewes reacted specifically with several antigens of EB as determined by one-dimensional (1-D) and 2-D gel electrophoresis; reactive antigens identified by mass spectrometry included the major outer membrane protein (MOMP), polymorphic outer membrane protein (POMP), and macrophage infectivity potentiator (MIP) lipoprotein.