An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata
An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata
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凋亡诱导剂阿拉林 (aralin) 是一种来自龙牙楤木 (Aralia elata) 的新型 II 型核糖体失活蛋白
DOI：
10.1515/bc.2004.107
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发表时间：
2004
影响因子：
2.9
通讯作者：
F. Tashiro
中科院分区：
文献类型：
--
作者：
M. Tomatsu;T. Kondo;T. Yoshikawa;T. Komeno;Naomi Adachi;Yasushi Kawasaki;A. Ikuta;F. Tashiro
摘要我们最近发现，一种由两个亚基组成的新型细胞毒蛋白aralin在转化细胞中选择性诱导细胞凋亡，而非正常细胞。本文报道了一种针对半乳糖（Gal）及其衍生物的凝集素，并作为一种新型II型核糖体失活蛋白（RIP）具有RNA n -糖苷酶活性。通过28S rRNA生成r片段，检测了无细胞和全细胞系统中aralin的RNA n -糖苷酶活性。与aralin处理的细胞中r片段的出现一致，在凋亡开始之前观察到显著的蛋白质合成抑制。添加Gal及其衍生物可有效抑制aralin引起的细胞死亡。有趣的是，与转化细胞相比，美利二糖优先保护正常细胞免于凋亡。罗丹明偶联的aralin可以在转化细胞的细胞表面周围清晰地检测到aralin受体，但在正常细胞表面的检测程度较低。这些结果表明，大蒜素通过其含有Gal的细胞表面受体进入细胞，并通过其RIP活性诱导细胞凋亡。此外，aralin受体的表达水平和/或结构变化可能影响对aralin的敏感性。
Abstract We recently found that aralin, a novel cytotoxic protein consisting of two subunits, from Aralia elata selectively induces apoptosis in transformed cells as compared to normal cells. Here we report that aralin is a lectin specific for galactose (Gal) and its derivatives, and possesses RNA N-glycosidase activity as a new type II ribosome-inactivating protein (RIP). The RNA N-glycosidase activity of aralin was detected in cell-free and whole cell systems by the generation of an R-fragment from 28S rRNA. Coinciding with appearance of the R-fragment in aralin-treated cells, significant inhibition of protein synthesis was observed prior to the onset of apoptosis. Aralin-evoked cell death was efficiently repressed by the addition of Gal and its derivatives. Interestingly, melibiose preferentially protected normal cells from apoptosis as compared with transformed cells. Using rhodamine-coupled aralin, the aralin receptor could be clearly detected around the cell surface of transformed cells, but to a lesser extent on normal cells. Receptor binding was suppressed by Gal. These results indicate that aralin is incorporated into cells via its Gal-containing cell surface receptor and induces apoptosis through its RIP activity. Moreover, the expression level and/or structural changes of the aralin receptor may affect the sensitivity toward aralin.
影响因子：
2.9
作者：
HARDY, MR;TOWNSEND, RR;LEE, YC
通讯作者：
LEE, YC