Differential Proteomic Analysis to Identify Proteins Associated with Apomeiosis in Boehmeria tricuspis (Hance) Makino Using an iTRAQ-Based Strategy
Differential Proteomic Analysis to Identify Proteins Associated with Apomeiosis in Boehmeria tricuspis (Hance) Makino Using an iTRAQ-Based Strategy
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使用基于 iTRAQ 的策略进行差异蛋白质组分析，鉴定与 Boehmeria tricuspis (Hance) Makino 的减数分裂相关的蛋白质
DOI：
10.1021/acs.jproteome.0c00586
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Jianguang Su
中科院分区：
文献类型：
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作者：
Qing Tang;Ying Xu;Canhui Deng;Chaohua Cheng;Zhigang Dai;Zemao Yang;Xiaojun Chen;Chan Liu;Jianguang Su
通过转录组学已经鉴定出许多与无融合生殖相关的候选基因，然而，无融合生殖的分子机制仍不清楚。阐明其潜在机制对于扩大其在作物育种中的应用至关重要。因此，在这里，我们采用同量异位素标签的相对和绝对定量标记技术，调查在功能大孢子阶段，通过不同的生殖方式产生的苎麻tricuspis蛋白质表达。我们鉴定了40个与无减数分裂相关的差异丰度蛋白，其中大部分参与了“应激反应”。功能分析表明，较低水平的活性氧（ROS）在诱导无减数分裂的发展中发挥作用。与ROS调节、细胞壁修饰和热胁迫下的稳定性相关的蛋白质在二倍体孢子无减数分裂的发育中起着至关重要的作用。我们的研究结果提供了证据的洞察力，压力可以诱导开关从无融合生殖性的ROS含量，和增加的组成的胁迫耐受性以及次生代谢产物可以缓冲ROS的影响。这些蛋白质参与相互关联的调控机制的精确协调可能在有性生殖向无融合生殖发育的过渡中共同起作用。
Numerous candidate genes related to apomixis have been identified through transcriptomics; however, the molecular mechanism underlying apomixis remains unclear. Elucidation of the underlying mechanisms is essential to expand its application in crop breeding. Therefore, here, we employed the isobaric tags for a relative and absolute quantification labeling technology to investigate the protein expression in Boehmeria tricuspis generated through different reproductive modes at the functional megaspore stage. We identified 40 differential abundance proteins associated with apomeiosis, most of which were involved in “response to stress”. Functional analysis suggested that lower levels of reactive oxygen species (ROS) play a role in inducing the development of apomeiosis. Proteins related to ROS regulation, cell wall modifications, and stability under heat stress play a crucial role in the development of diplosporic apomeiosis. Our results give evidence to the insight that stress can induce a switch from apomixis to sexuality by ROS content, and an increased composition of stress tolerance as well as secondary metabolites can buffer ROS effects. Precise coordination of these proteins involved in inter-related regulatory control mechanisms may act together in the transition from the sexual to apomixis development.