MicroRNA-874 inhibits cell proliferation and induces apoptosis in human breast cancer by targeting CDK9
MicroRNA-874 inhibits cell proliferation and induces apoptosis in human breast cancer by targeting CDK9
复制标题
MicroRNA-874 通过靶向 CDK9 抑制人乳腺癌细胞增殖并诱导细胞凋亡
DOI：
10.1016/j.febslet.2014.09.035
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发表时间：
2014-12-20
期刊：
影响因子：
3.5
通讯作者：
Wang, Fuqiang
中科院分区：
文献类型：
--
作者：
Wang, Ling;Gao, Wen;Wang, Fuqiang
已经证明miR-874在许多类型的癌症中起重要作用。然而，其在乳腺癌中的生物学功能在很大程度上仍然未知。在本研究中，我们发现miR-874在乳腺癌中的表达水平与癌旁正常组织相比下调。miR-874的过表达能够抑制MCF 7和MDA-MB-231细胞的细胞增殖并诱导细胞凋亡和细胞周期停滞。使用生物信息学方法，我们进一步表明CDK 9是miR-874的直接靶点，并且其蛋白水平受miR-874负调控。因此，本文报道的数据表明miR-874是乳腺癌中的重要调节因子，并暗示miR-874/CDK 9轴具有作为乳腺癌治疗靶点的潜力。(C)2014年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
It has been demonstrated that miR-874 plays important roles in many types of cancers. Nevertheless, its biological function in breast cancer remains largely unknown. In this study, we found that the expression level of miR-874 is down-regulated in breast cancer in comparison with the adjacent normal tissues. The overexpression of miR-874 is able to inhibit cell proliferation and induce cell apoptosis and cell cycle arrest in MCF7 and MDA-MB-231 cells. Using a bioinformatics method, we further show that CDK9 is a direct target of miR-874 and that its protein level is negatively regulated by miR-874. Therefore, the data reported in this manuscript demonstrate that miR-874 is an important regulator in breast cancer and imply that the miR-874/CDK9 axis has potential as a therapeutic target for breast cancer. (C) 2014 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.