HEPATITIS-B VIRUS-DNA IN PATIENTS WITH CHRONIC LIVER-DISEASE AND NEGATIVE TESTS FOR HEPATITIS-B SURFACE-ANTIGEN
HEPATITIS-B VIRUS-DNA IN PATIENTS WITH CHRONIC LIVER-DISEASE AND NEGATIVE TESTS FOR HEPATITIS-B SURFACE-ANTIGEN
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DOI：
10.1056/nejm198501313120503
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发表时间：
1985-01-01
影响因子：
158.5
通讯作者：
BERTHELOT, P
中科院分区：
文献类型：
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作者：
BRECHOT, C;DEGOS, F;BERTHELOT, P
对134例B型肝炎表面抗原（HBsAg）阴性慢性肝病（包括20例肝细胞癌）患者的肝脏或血清标本中存在的B型肝炎病毒（HBV）DNA进行了检测。88份肝组织标本中有52份（59%）检出HBV DNA序列，其中20份肝细胞癌标本中有17份检出HBV DNA序列。在88份肝脏样本中仅5份检测到HBV DNA的复制形式，其中3份来自无HBV血清学标志物的患者。在大多数肝脏样本中，DNA模式与HBV或密切相关病毒的存在一致。105份血清标本中，10份（9.5%）检出HBV DNA序列，其中6份无HBV血清学标志。17例HBV DNA阳性患者中有5例检测到HBsAg相关决定因素，14例阴性患者中无一检测到HBsAg相关决定因素。高频率的HBsAg阴性的HBV DNA阳性的病毒感染的肝脏被证明;和HBV的增殖可能会发生在没有任何常规的HBV血清学标志物。
The presence of hepatitis B virus (HBV) DNA in liver or serum samples from 134 patients with hepatitis B surface antigen (HBsAg)-negative chronic liver disease, including 20 with hepatocellular carcinoma. HBV DNA sequences were detected in 52 of the 88 liver samples (59%), including 17 of the 20 samples from patients with hepatocellular carcinoma. Presumably replicative forms of HBV DNA were detected in only 5 of the 88 liver samples, 3 of which were from patients with no serologic marker for HBV. In most of the liver samples the DNA patterns were consistent with the presence of HBV or a closely related virus. Of the 105 serum samples tested, HBV DNA sequences were identified in 10 (9.5%), 6 of which had no HBV serologic marker. HBsAg-associated determinants were detected in 5 of 17 patients who were positive for HBV DNA and in none of 14 patients who were negative. The high frequency of HBsAg-negative HBV DNA-positive viral infection of the liver was demonstrated; and multiplication of HBV may occur in the absence of any conventional serologic marker for HBV.