Perkinsus mediterraneus n. sp., a protistan parasite of the European flat oyster Ostrea edulis from the Balearic Islands, Mediterranean Sea
Perkinsus mediterraneus n. sp., a protistan parasite of the European flat oyster Ostrea edulis from the Balearic Islands, Mediterranean Sea
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DOI：
10.3354/dao058231
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发表时间：
2004-03-10
影响因子：
1.4
通讯作者：
Villalba, A
中科院分区：
文献类型：
--
作者：
Casas, SM;Grau, A;Villalba, A
本文报道了寄生于欧洲牡蛎Ostrea edulis（L.）养殖沿着的巴利阿里群岛，地中海，描述。形态学检查，光镜和透射电镜，DNA序列分析和扩大射线的流体巯基乙酸盐培养基（RFTM）证实，这种寄生虫属于属帕金苏斯。小亚基核糖体（ssu rRNA）基因测序结果表明，该虫属于帕金虫属（Perkinsus），其ITS序列与已发表的帕金虫ITS序列完全不同。系统发育分析表明，露种的ITS序列形成了一个单系群，包括一个姐妹分支的P. atlanticus/olseni组。此外，观察到新物种与其他已描述的Perkinsus spp.之间的形态差异。在RFTM中孵育1周后，prezoosporangiurn已达到非常大的尺寸（97.4 +/- 1.99 μ m）（平均值+/- SE），孵育2周后，尺寸再次几乎加倍（167.1 +/- 8.09 μ m）。放电管的长度是游动孢子囊直径的六分之一，即17.36：97.38的比例，是所有帕金虫属物种中观察到的最低比例。在超微结构水平上，游动孢子囊和游动孢子与其他帕金虫属物种相比表现出一些差异。
A new species, Perkinsus mediterraneus, a protistan parasite of the European oyster Ostrea edulis (L.), farmed along the coast of the Balearic Islands, Mediterranean Sea, is described. Morphological examinations with light and transmission electron microscopy, DNA sequence-analysis and enlargement in Ray's fluid thioglycollate medium (RFTM) confirmed that this parasite belongs to the genus Perkinsus. Specific morphological and genetic characteristics indicated that it should be considered a new species in the genus, Sequencing of the small subunit ribosomal (ssu rRNA) gene confirmed that the parasite belongs to the genus Perkinsus, and sequences of the internal transcribed spacer (ITS) were distinct from any Perkinsus ITS sequences previously published and/or deposited in the GenBank. Phylogenetic analysis revealed that the ITS sequences of the Dew species formed a monophyletic group comprising a sister clade to the P. atlanticus/olseni group. In addition, morphological differences were observed between the new species and the other described Perkinsus spp.. After incubation in RFTM for 1 wk, the prezoosporangiurn had reached an extremely large size (97.4 +/- 1.99 mum) (mean +/- SE), and after 2 wk incubation had again almost doubled in size (167.1 +/- 8.09 mum). The discharge-tube length was one sixth the diameter of the zoosporangium, i.e. a ratio of 17.36:97.38, the lowest ratio observed for any Perkinsus species. At the ultrastructural level, zoosporangia and zoospores exhibited some differences compared to other Perkinsus species.