Mapping of rat Chromosome 2 markers generated from chromosome-sorted DNA
Mapping of rat Chromosome 2 markers generated from chromosome-sorted DNA
复制标题
从染色体分选的 DNA 生成的大鼠 2 号染色体标记图谱
作者：
A. Deng;Clara M Jackson;B. Hoebee;J. Rapp
摘要。最近，通过连锁和使用同源菌株，在大鼠染色体（Chr） 2上定位了一个大约30 cM的血压数量性状位点（QTL）。然而，为了进一步精确定位QTL，需要在这个30厘米区域内的紧密连锁染色体标记。在目前的工作中，通过筛选大鼠Chr 2排序的DNA文库产生了36个新的标记，并随后使用5个F2群体进行了定位。结合新标记和现有标记，30 cM区域的标记密度接近平均每1.1 cM 1个标记。
Abstract. A quantitative trait locus (QTL) for blood pressure has recently been mapped to a region of roughly 30 cM on rat Chromosome (Chr) 2 by linkage and by the use of congenic strains. For further fine mapping of the QTL, however, closely linked chromosome markers residing in this 30-cM region are required. In the current work, 36 new markers were generated by screening rat Chr 2-sorted DNA libraries and subsequently mapped using five F2 populations. Combining new and existing markers, the marker density for the 30-cM region approaches, on average, one marker per 1.1 cM.