Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum.
Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum.
复制标题
利用 RNA 干扰敲低 5 个海藻糖酶基因调节赤拟谷盗几丁质生物合成途径的基因表达
DOI：
10.1186/s12896-016-0297-2
复制
发表时间：
2016-09-06
影响因子：
3.5
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Tang B;Wei P;Zhao L;Shi Z;Shen Q;Yang M;Xie G;Wang S
RNA干扰是研究基因功能的一种非常有效的方法，并且可能是控制害虫的有效方法。海藻糖酶是昆虫几丁质生物合成途径的关键基因。赤拟谷盗中已克隆了五个海藻糖酶基因，但尚不清楚这些海藻糖酶的详细功能是否可用于害虫防治。使用RNAi研究了所有5个海藻糖酶基因的功能，最重要的结果表明，与对照组相比，所有12个基因的表达在12至72小时内均显着下降，除了72小时时的GP1，此时TcTre2基因的表达受到抑制。结果还揭示了不同的异常表型，观察到的死亡率范围为 17% 至 42%。 qRT-PCR结果显示，沉默5种海藻糖酶48 h后，RNAi后TPS、GS、2个GP、CHS1a和CHS1b基因的表达量显着下降，而CHS2基因的表达量下降或上升。此外，注射 dsTcTre 后 12 小时至 72 小时，TPS 基因表达下降。这些结果表明，任何单个海藻糖酶基因的沉默，特别是Tre1-4和Tre2基因，都可以通过几丁质生物合成途径中基因表达的调节导致换羽畸形和高死亡率，并且可能是未来害虫防治的潜在方法。本文的在线版本 (doi:10.1186/s12896-016-0297-2) 包含补充材料，可供授权用户使用。
RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five trehalase genes have been cloned in Tribolium castaneum, though it is not known whether the detailed functions of these trehalases can be targeted for pest control. The functions of all five trehalase genes were studied using RNAi, and the most important results showed that the expression of all 12 genes decreased significantly from 12 to 72 h compared with the control groups, except GP1 at 72 h, when the expression of the TcTre2 gene was suppressed. The results also revealed different abnormal phenotypes, and the observed mortality rates ranged from 17 to 42 %. The qRT-PCR results showed that the expression of TPS, GS, two GP, CHS1a and CHS1b genes decreased significantly, while that of the CHS2 gene decreased or increased after RNAi after the five trehalases were silenced at 48 h. In addition, TPS gene expression decreased from 12 to 72 h after dsTcTre injection. These results demonstrate that silencing of any individual trehalase gene, especially Tre1-4 and Tre2 gene can lead to moulting deformities and a high mortality rate through the regulation of gene expression in the chitin biosynthesis pathway and may be a potential approach for pest control in the future. The online version of this article (doi:10.1186/s12896-016-0297-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
RNAi在豌豆蚜虫acyrthosiphon pisum中敲除基因。
影响因子：
3.5
作者：
Jaubert-Possamai, Stephanie;Le Trionnaire, Gael;Bonhomme, Joel;Christophides, Georges K;Rispe, Claude;Tagu, Denis
通讯作者：
Tagu, Denis
影响因子：
56.9
作者：
CLEGG, JS;EVANS, DR
通讯作者：
EVANS, DR
影响因子：
2.2
作者：
Kamei, Yuhki;Hasegawa, Yuh;Yaginuma, Toshinobu
通讯作者：
Yaginuma, Toshinobu
影响因子：
1.6
作者：
de Almeida, Fabiana M.;Bonini, Beatriz M.;da Silva, Aline M.
通讯作者：
da Silva, Aline M.
影响因子：
2.6
作者：
Kunieda T;Fujiyuki T;Kucharski R;Foret S;Ament SA;Toth AL;Ohashi K;Takeuchi H;Kamikouchi A;Kage E;Morioka M;Beye M;Kubo T;Robinson GE;Maleszka R
通讯作者：
Maleszka R