Utilization of Microbial Biofilms as Monitors of Bioremediation
Utilization of Microbial Biofilms as Monitors of Bioremediation
复制标题
利用微生物生物膜作为生物修复的监测器
DOI：
10.1007/s00248-003-1024-9
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发表时间：
2004
影响因子：
3.6
通讯作者：
David C. White
中科院分区：
文献类型：
--
作者：
A. Peacock;Yun;J. Istok;L. Krumholz;Roland Geyer;B. Kinsall;David B. Watson;K. Sublette;David C. White
使用玻璃棉或 Bio-Sep 珠作为固相支持基质开发了井下含水层微生物采样系统。在这里，我们描述了在美国能源部橡树岭国家实验室自然和加速生物修复研究计划现场研究中心进行现场生物修复实验期间，如何使用这些设备来监测地下水微生物群落动态。在为期 6 周的部署过程中，微生物生物膜在玻璃棉和珠子的内表面上定殖。活生物量、群落组成、代谢状态和呼吸状态的变化反映在采样器组成、供体类型和地下水 pH 值中。 Bio-Sep 珠上形成的生物膜的活生物量增加了 2-13 倍；然而，与玻璃棉上形成的生物膜相比，珠群落的代谢活性较低[较高的环丙烷/单烯磷脂脂肪酸（PLFA）比率]并且具有较低的有氧呼吸状态（较低的总呼吸醌/PLFA比率和泛醌/甲基萘醌比率）。这些系统中的厌氧生长以缩醛磷脂为特征，并且在接受电子供体添加的孔中生长得更快。部分 16S rDNA 序列表明，添加乙酸盐、乙醇或葡萄糖可诱导地杆菌和硝酸盐还原生物体。 DNA 和脂质生物标记物的提取和回收不会出现因粘土或溶解有机物的存在而经常困扰沉积物样品的并发症。尽管地下水或邻近沉积物中的微生物群落组成可能与井下生物膜采样器上形成的微生物群落组成不同，但生物膜对地下水地球化学变化的代谢活动响应记录了微生物群落对生物刺激的响应，同时为生物标志物分析提供了综合采样和易于恢复。
A down-well aquifer microbial sampling system was developed using glass wool or Bio-Sep beads as a solid-phase support matrix. Here we describe the use of these devices to monitor the groundwater microbial community dynamics during field bioremediation experiments at the U.S. Department of Energy Natural and Accelerated Bioremediation Research Program’s Field Research Center at the Oak Ridge National Laboratory. During the 6-week deployment, microbial biofilms colonized glass wool and bead internal surfaces. Changes in viable biomass, community composition, metabolic status, and respiratory state were reflected in sampler composition, type of donor, and groundwater pH. Biofilms that formed on Bio-Sep beads had 2–13 times greater viable biomass; however, the bead communities were less metabolically active [higher cyclopropane/monoenoic phospholipid fatty acid (PLFA) ratios] and had a lower aerobic respiratory state (lower total respiratory quinone/PLFA ratio and ubiquinone/menaquinone ratio) than the biofilms formed on glass wool. Anaerobic growth in these systems was characterized by plasmalogen phospholipids and was greater in the wells that received electron donor additions. Partial 16S rDNA sequences indicated that Geobacter and nitrate-reducing organisms were induced by the acetate, ethanol, or glucose additions. DNA and lipid biomarkers were extracted and recovered without the complications that commonly plague sediment samples due to the presence of clay or dissolved organic matter. Although microbial community composition in the groundwater or adjacent sediments may differ from those formed on down-well biofilm samplers, the metabolic activity responses of the biofilms to modifications in groundwater geochemistry record the responses of the microbial community to biostimulation while providing integrative sampling and ease of recovery for biomarker analysis.
DOI：
10.1099/00207713-44-2-338
发表时间：
1994-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
MOORE, LVH;BOURNE, DM;MOORE, WEC
通讯作者：
MOORE, WEC