Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa.
Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa.
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DOI：
10.15698/mic2014.09.165
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发表时间：
2014-08-09
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影响因子：
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通讯作者：
Videira A
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作者：
Gonçalves AP;Monteiro J;Lucchi C;Kowbel DJ;Cordeiro JM;Correia-de-Sá P;Rigden DJ;Glass NL;Videira A
细胞内钙水平的改变是动物和真菌对细胞死亡刺激的常见反应，特别是粗糙脉孢菌对星形孢菌素的反应。我们强调了细胞外Ca2+的可用性对这种反应的重要性。培养基中离子的限制进一步使细胞对药物敏感，并导致活性氧（ROS）的积累增加。相反，大约30倍过量的外部Ca2+导致药物耐受性增加和ROS产生减少。与此一致，在不存在或存在过量外部Ca2+的情况下观察到不同的星形孢菌素诱导的胞质Ca2+信号传导谱。高通量RNA测序显示，不同浓度的细胞外Ca2+定义不同的转录程序。我们的转录谱还指出了两个推定的新的钙离子结合蛋白，编码的NCU 08524和NCU 06607基因，并提供了一个参考数据集，为今后的调查中的作用，钙离子在真菌生物学。
Alterations in the intracellular levels of calcium are a common response to cell death stimuli in animals and fungi and, particularly, in the Neurospora crassa response to staurosporine. We highlight the importance of the extracellular availability of Ca2+ for this response. Limitation of the ion in the culture medium further sensitizes cells to the drug and results in increased accumulation of reactive oxygen species (ROS). Conversely, an approximately 30-fold excess of external Ca2+ leads to increased drug tolerance and lower ROS generation. In line with this, distinct staurosporine-induced cytosolic Ca2+ signaling profiles were observed in the absence or presence of excessive external Ca2+. High-throughput RNA sequencing revealed that different concentrations of extracellular Ca2+ define distinct transcriptional programs. Our transcriptional profiling also pointed to two putative novel Ca2+-binding proteins, encoded by the NCU08524 and NCU06607 genes, and provides a reference dataset for future investigations on the role of Ca2+ in fungal biology.