Male Microchimerism in the Human Female Brain
Male Microchimerism in the Human Female Brain
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DOI：
10.1371/journal.pone.0045592
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发表时间：
2012-09-26
期刊：
影响因子：
3.7
通讯作者：
Nelson, J. Lee
中科院分区：
文献类型：
--
作者：
Chan, William F. N.;Gurnot, Cecile;Nelson, J. Lee
在人类中，已经在许多组织和器官中观察到自然获得的微嵌合体。然而，胎儿微嵌合体尚未在人脑中进行研究。最近有报道在小鼠脑中存在胎儿和母体来源的微嵌合体。在这项研究中，我们量化了人类女性大脑中的男性DNA，作为胎儿来源的微嵌合体的标志物（即女性在怀男性胎儿时获得男性DNA）。针对Y染色体特异性DYS14基因，我们在没有神经系统疾病临床或病理证据的女性（n = 26）或患有阿尔茨海默病的女性（n = 33）的尸检大脑中进行实时定量PCR。我们报告说，63%的女性（37 59）测试窝藏男性微嵌合体在大脑中。雄性微嵌合体存在于多个脑区。结果还表明，与无神经系统疾病的女性相比，阿尔茨海默病女性大脑中男性微嵌合体的患病率（p = 0.03）和浓度（p = 0.06）较低。总之，男性微嵌合体是频繁和广泛分布在人类女性大脑。
In humans, naturally acquired microchimerism has been observed in many tissues and organs. Fetal microchimerism, however, has not been investigated in the human brain. Microchimerism of fetal as well as maternal origin has recently been reported in the mouse brain. In this study, we quantified male DNA in the human female brain as a marker for microchimerism of fetal origin (i.e. acquisition of male DNA by a woman while bearing a male fetus). Targeting the Y-chromosome-specific DYS14 gene, we performed real-time quantitative PCR in autopsied brain from women without clinical or pathologic evidence of neurologic disease (n = 26), or women who had Alzheimer's disease (n = 33). We report that 63% of the females (37 of 59) tested harbored male microchimerism in the brain. Male microchimerism was present in multiple brain regions. Results also suggested lower prevalence (p = 0.03) and concentration (p = 0.06) of male microchimerism in the brains of women with Alzheimer's disease than the brains of women without neurologic disease. In conclusion, male microchimerism is frequent and widely distributed in the human female brain.