Characterization of the growth of cultured cortical neurons on sections of adult rat cortex.
Characterization of the growth of cultured cortical neurons on sections of adult rat cortex.
复制标题
成年大鼠皮质切片上培养的皮质神经元生长的表征。
DOI：
10.1093/cercor/1.2.134
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
GeisertJr,EE
中科院分区：
文献类型：
--
作者：
GeisertJr,EE
将从胚胎第18天（E18）大鼠皮层分离的培养神经元接种到成年CNS和外周神经的低温恒温切片上。将这些切片支持神经元附着和神经突延伸的能力与相邻的聚-L-赖氨酸（PLL）包被的玻璃盖玻片上的神经元的能力进行比较。数据的定量分析表明，大脑皮层的部分提供了一个很好的基板神经元的附着和生长的神经突，类似于PLL涂层的玻璃盖玻片。与皮质切片相比，坐骨神经切片支持神经元附着和突起生长的能力降低。视神经的切片支持有限的神经元附着，并且存在于切片顶部的少数神经元没有突起。对照实验表明，皮层切片支持培养的神经元的附着和生长的能力是由于切片的分子组成，而不是组织培养环境的影响。神经元与CNS皮质切片的相互作用部分依赖于存在于培养神经元上的细胞外基质受体β 1整合素家族的成员。该体外系统允许定义生长的轴突和成人皮质环境之间可能发生的一些分子和细胞相互作用。
Cultured neurons isolated from the embryonic day 18 (E18) rat cortex were plated onto cryostat sections of adult CNS and peripheral nerve. The ability of these sections to support neuronal attachment and neurite extension was compared to that of neurons on the adjacent poly-L-lysino (PLL)-coated glass coverslips. A quantitative analysis of the data demonstrated that sections of cerebral cortex provide a good substrate for neuronal attachment and growth of neurites, similar to that of the PLL-coated glass coverslips. Sections of sciatic nerve demonstrated a decreased ability to sup port neuronal attachment and the growth of processes as compared to sections of cortex. Sections of opticnerve supported limited neuronal attachment, and the few neurons that were present on top of the sections were devoid of processes. Control experiments showed that the ability of the cortical sections to support the attachment and growth of cultured neurons was due to the molecular composition of the sections and not to influences of the tissue culture environment. The interactions of neurons with the sections of CNS cortex were partially dependent on a member of the β1integrin family of extracellular matrix receptors present on cultured neurons. Thisin vitrosystem allowed for the definition of some of the molecular and the cellular interactions that may occur between growing axons and the environment of the adult cortex.