In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules.
In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules.
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DOI：
10.1016/j.ymeth.2016.04.032
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发表时间：
2016-08-15
期刊：
影响因子：
--
通讯作者：
Stojanovic MN
中科院分区：
文献类型：
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作者：
Yang KA;Pei R;Stojanovic MN
我们最近优化了一种程序，可以以所谓的结构转换形式直接产生小分子的适配体传感器。该协议成功率高，时间短，并且足够简单，可以在非专业实验室中轻松实施。我们为该选择协议提供了逐步指南。
We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol.